ArticleJournal of clinical microbiology2002
Detection of methicillin resistance in primary blood culture isolates of coagulase-negative staphylococci by PCR, slide agglutination, disk diffusion, and a commercial method.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 21 citations in OpenAlex.
- Expert systems in clinical microbiology.Clinical microbiology reviews · 2011Review
- Comparison of the BD Phoenix system with the cefoxitin disk diffusion test for detection of methicillin resistance in Staphylococcus aureus and coagulase-negative staphylococci.Journal of clinical microbiology · 2009Article
- Comparison of conventional susceptibility testing, penicillin-binding protein 2a latex agglutination testing, and mecA real-time PCR for detection of oxacillin resistance in Staphylococcus aureus and coagulase-negative Staphylococcus.Journal of clinical microbiology · 2005Article
- Shortcut method for extraction of Staphylococcus aureus DNA from blood cultures and conventional cultures for use in real-time PCR assays.Journal of clinical microbiology · 2005Article
- Evaluation of the BD PHOENIX automated microbiology system for detection of methicillin resistance in coagulase-negative staphylococci.Journal of clinical microbiology · 2004Article
- Coagulase-negative staphylococci: comparison of phenotypic and genotypic oxacillin susceptibility tests and evaluation of the agar screening test by using different concentrations of oxacillin.Journal of clinical microbiology · 2003Article
- Two-center collaborative evaluation of the performance of the BD Phoenix automated microbiology system for identification and antimicrobial susceptibility testing of Enterococcus spp. and Staphylococcus spp.Journal of clinical microbiology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The methicillin resistance of 363 coagulase-negative staphylococci isolated from blood cultures was determined by a slide latex agglutination (LA) test for penicillin-binding protein 2a (PBP 2a), the presence of the mecA gene by PCR, disk diffusion, and Vitek. LA was performed on primary cultures, and PBP 2a expression was induced by placing an oxacillin disk in the primary inoculum. Compared to the PCR results, LA was the most sensitive and specific in the detection of methicillin resistance. Without induction, LA failed to detect 50% of mecA-positive strains grown on two different media.
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Registered trials
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