ArticleJournal of clinical microbiology2002
Automated extraction of genomic DNA from medically important yeast species and filamentous fungi by using the MagNA Pure LC system.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Polymerase chain reaction blood tests for the diagnosis of invasive aspergillosis in immunocompromised people.The Cochrane database of systematic reviews · 2019Pooled it
- Randomized PCR-based therapy and risk factors for invasive fungal infection following reduced-intensity conditioning and hematopoietic SCT.Bone marrow transplantation · 2010Trial
- Isolation and molecular characterization of mycotoxigenic fungi in agarwood.Saudi journal of biological sciences · 2018Article
- Determining fungi rRNA copy number by PCR.Journal of biomolecular techniques : JBT · 2013Article
- Genotyping 1000 yeast strains by next-generation sequencing.BMC genomics · 2013Article
- The era of molecular and other non-culture-based methods in diagnosis of sepsis.Clinical microbiology reviews · 2010Review
- Detection of a molecular biomarker for zygomycetes by quantitative PCR assays of plasma, bronchoalveolar lavage, and lung tissue in a rabbit model of experimental pulmonary zygomycosis.Journal of clinical microbiology · 2008Article
- Automated and manual methods of DNA extraction for Aspergillus fumigatus and Rhizopus oryzae analyzed by quantitative real-time PCR.Journal of clinical microbiology · 2008Article
- A consensus on fungal polymerase chain reaction diagnosis?: a United Kingdom-Ireland evaluation of polymerase chain reaction methods for detection of systemic fungal infections.The Journal of molecular diagnostics : JMD · 2006Article
- Evaluation of molecular diagnostic assays for fungal infections.The Journal of molecular diagnostics : JMD · 2006Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Development of a DNA microarray for detection and identification of fungal pathogens involved in invasive mycoses.Journal of clinical microbiology · 2005Article
- Assessment of ribosomal large-subunit D1-D2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important Aspergillus species.Journal of clinical microbiology · 2005Article
- A prospective study of real-time panfungal PCR for the early diagnosis of invasive fungal infection in haemato-oncology patients.Bone marrow transplantation · 2005Article
- Development of a method to detect and quantify Aspergillus fumigatus conidia by quantitative PCR for environmental air samples.Mycopathologia · 2004Article
- Rapid differentiation of Aspergillus species from other medically important opportunistic molds and yeasts by PCR-enzyme immunoassay.Journal of clinical microbiology · 2004Article
- Early detection and identification of commonly encountered Candida species from simulated blood cultures by using a real-time PCR-based assay.The Journal of molecular diagnostics : JMD · 2004Article
- Recent developments for diagnosis of toxoplasmosis.Journal of clinical microbiology · 2004Review
- Fully automated, internally controlled quantification of hepatitis B Virus DNA by real-time PCR by use of the MagNA Pure LC and LightCycler instruments.Journal of clinical microbiology · 2004Article
- Pulmonary aspergillosis in a patient with chronic granulomatous disease: confirmation by polymerase chain reaction and serological tests, and successful treatment with voriconazole.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2003Article
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Authors and funding
5 authors.
Funding
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Abstract
A fully automated assay was established for the extraction of DNA from clinically important fungi by using the MagNA Pure LC instrument. The test was evaluated by DNA isolation from 23 species of yeast and filamentous fungi and by extractions (n = 28) of serially diluted Aspergillus fumigatus conidia (10(5) to 0 CFU/ml). Additionally, DNA from 67 clinical specimens was extracted and compared to the manual protocol. The detection limit of the MagNA Pure LC assay of 10 CFU corresponded to the sensitivity when DNA was extracted manually; in 9 of 28 runs, we could achieve a higher sensitivity of 1 CFU/ml blood, which was found to be significant (p <or= 0.004). DNA from all fungal species analyzed could be extracted and amplified by real-time PCR. Negative controls from all MagNA Pure isolations remained negative. Sixty-three clinical samples showed identical results by both methods, whereas in 4 of 67 samples, discordant results were obtained. Thus, the MagNA Pure LC technique offers a fast protocol for automated DNA isolation from numerous fungi, revealing high sensitivity and purity.
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