ArticleJournal of clinical microbiology2002
Genotyping of hepatitis C virus types 1, 2, 3, and 4 by a one-step LightCycler method using three different pairs of hybridization probes.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Genotyping & diagnostic methods for hepatitis C virus: A need of low-resource countries.The Indian journal of medical research · 2018Review
- Monitoring the Transcriptional Activity of Human Endogenous Retroviral HERV-W Family Using PNA Strand Invasion into Double-Stranded DNA.Molecular biotechnology · 2018Article
- Molecular Assay and Genotyping of Hepatitis C Virus among Infected Egyptian and Saudi Arabian Patients.Virology : research and treatment · 2015Article
- Hepatitis C virus genotype 6: virology, epidemiology, genetic variation and clinical implication.World journal of gastroenterology · 2014Review
- Introduction of an automated system for the diagnosis and quantification of hepatitis B and hepatitis C viruses.The open virology journal · 2012Article
- Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C.Virology journal · 2011Article
- Development of hepatitis C virus genotyping by real-time PCR based on the NS5B region.PloS one · 2010Article
- Multiplex real-time reverse transcription-PCR assay for determination of hepatitis C virus genotypes.Journal of clinical microbiology · 2006Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Hepatitis C genotyping by denaturing high-performance liquid chromatography.Journal of clinical microbiology · 2004Article
- Clinical evaluation of two methods for genotyping hepatitis C virus based on analysis of the 5' noncoding region.Journal of clinical microbiology · 2003Article
- Subtyping of hepatitis C virus with high resolution mass spectrometry.Clinical mass spectrometry (Del Mar, Calif.)Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Determination of hepatitis C virus (HCV) genotypes has become increasingly important during the last years for prediction of the clinical course and the outcome of antiviral therapy. Therefore, numerous different methods have been developed to enable HCV genotyping. However, many of them are very laborious and expensive, leading to limited usage in daily routine diagnostics. We have established a method which combines the speed of the new LightCycler technology with the use of amplification products generated for diagnostic quantitative HCV RNA determination. Differentiation of HCV genotypes is performed with these amplicons in a single step by using fluorophore-labeled hybridization probes. Although currently only two different acceptor fluorophores are available for the LightCycler, types 1, 2, 3, and 4, which are by far the prevailing HCV genotypes in Europe and the United States, can be distinguished. Genotypes of specimens from 190 chronically HCV-infected patients were determined by the LightCycler method and compared with the results of nucleotide sequencing. Concordant results were obtained for all samples. This new method offers a fast and convenient possibility to determine the quantitative HCV RNA load and the genotype in large-scale settings within about 4 h.
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