ArticleInvestigative ophthalmology & visual science2002
Identification of differentially expressed genes in mouse Pax6 heterozygous lenses.
Article in Investigative ophthalmology & visual science, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed, 36 citations in OpenAlex.
- Deep mutational scanning quantifies DNA binding and predicts clinical outcomes of PAX6 variants.Molecular systems biology · 2024Article
- Autoregulation of Pax6 in neuronal cells is mediated by Pax6(5a), Pax6(ΔPD), SPARC, and p53.Molecular biology reports · 2022Article
- Pax6 influences expression patterns of genes involved in neuro- degeneration.Annals of neurosciences · 2015Article
- Whole exome sequence analysis of Peters anomaly.Human genetics · 2014Article
- Regulation of mouse lens maturation and gene expression by Krüppel-like factor 4.Experimental eye research · 2013Article
- Discovery and assessment of conserved Pax6 target genes and enhancers.Genome research · 2011Article
- Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila.Developmental biology · 2008Article
- Eyg and Ey Pax proteins act by distinct transcriptional mechanisms in Drosophila development.The EMBO journal · 2005Article
- Regulation of gene expression by Pax6 in ocular cells: a case of tissue-preferred expression of crystallins in lens.The International journal of developmental biology · 2004Review
- Functional interactions between alternatively spliced forms of Pax6 in crystallin gene regulation and in haploinsufficiency.Nucleic acids research · 2004Article
- A comparative cDNA microarray analysis reveals a spectrum of genes regulated by Pax6 in mouse lens.Genes to cells : devoted to molecular & cellular mechanisms · 2002Article
- Building the developmental oculome: systems biology in vertebrate eye development and disease.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposePax6 is a critical regulator of the developing lens, other ocular tissues, central nervous system, and pancreas. Downstream targets of Pax6 are largely unknown. The present study was designed to identify differentially expressed genes in Pax6 heterozygous and normal mouse lenses.
methodsRNAs from 8-week-old normal and Pax6 heterozygous mouse lenses were analyzed by both RT-PCR differential display and a candidate-gene approach. The expression levels of identified genes were confirmed by semiquantitative RT-PCR.
resultsEight transcripts encoding phosphatase inhibitor Pip-1 protein, heat shock protein Hsp40, Purkinje cell protein Pcp4, an expressed sequence tag (EST; AA331381) originally reported in a brain-specific library, Pitx3, and CBP, were confirmed to be downregulated in the Pax6 heterozygous mouse lenses. alphaB- and betaA3/A1-crystallin transcripts exhibited decreased expression in Pax6 heterozygous lenses, whereas the expression levels of other crystallins were virtually unchanged.
conclusionsThe present data identify eight genes with expression levels that are decreased in Pax6 heterozygous lenses and provide evidence that four functional categories of transcripts-namely, small hsps (alphaB-crystallin and Hsp40), crystallins (alphaB- and betaA3/A1-crystallin), transcription factors (Pitx-3 and CBP), and components of signal transduction cascades (Pip-1) are under direct or indirect transcriptional control by Pax6.
Indexed as
Identifiers
12036994PMC2080866W1494106563What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.