ArticleBlood2002
Arp2/3 complex is required for actin polymerization during platelet shape change.
Article in Blood, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 67 citations in OpenAlex.
- Article
- Potential Biomarkers of Acute Ischemic Stroke Etiology Revealed by Mass Spectrometry-Based Proteomic Characterization of Formalin-Fixed Paraffin-Embedded Blood Clots.Frontiers in neurology · 2022Article
- Live-cell super-resolved PAINT imaging of piconewton cellular traction forces.Nature methods · 2020Article
- New insights into cytoskeletal remodeling during platelet production.Journal of thrombosis and haemostasis : JTH · 2019Review
- Integrin αBlood · 2017Review
- Sensitized mutagenesis screen in Factor V Leiden mice identifies thrombosis suppressor loci.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Deletion of the Arp2/3 complex in megakaryocytes leads to microthrombocytopenia in mice.Blood advances · 2017Article
- Balance of microtubule stiffness and cortical tension determines the size of blood cells with marginal band across species.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Non-genomic activities of retinoic acid receptor alpha control actin cytoskeletal events in human platelets.Journal of thrombosis and haemostasis : JTH · 2016Article
- Pdlim7 Regulates Arf6-Dependent Actin Dynamics and Is Required for Platelet-Mediated Thrombosis in Mice.PloS one · 2016Article
- Article
- Platelet Rho GTPases-a focus on novel players, roles and relationships.The Biochemical journal · 2015Review
- Proteomic analysis of differential protein expression in platelets of septic patients.Molecular biology reports · 2014Article
- p21 activated kinase signaling coordinates glycoprotein receptor VI-mediated platelet aggregation, lamellipodia formation, and aggregate stability under shear.Arteriosclerosis, thrombosis, and vascular biology · 2013Article
- Studies on the actin-binding protein HS1 in platelets.BMC cell biology · 2007Article
- Article
- Rac1 is essential for platelet lamellipodia formation and aggregate stability under flow.The Journal of biological chemistry · 2005Article
- The formin homology 1 domain modulates the actin nucleation and bundling activity of Arabidopsis FORMIN1.The Plant cell · 2005Article
- Signalling to actin assembly via the WASP (Wiskott-Aldrich syndrome protein)-family proteins and the Arp2/3 complex.The Biochemical journal · 2004Review
- Formation of filopodia-like bundles in vitro from a dendritic network.The Journal of cell biology · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Platelets undergo a series of actin-dependent morphologic changes when activated by thrombin receptor activating peptide (TRAP) or when spreading on glass. Polymerization of actin results in the sequential formation of filopodia, lamellipodia, and stress fibers, but the molecular mechanisms regulating this polymerization are unknown. The Arp2/3 complex nucleates actin polymerization in vitro and could perform this function inside cells as well. To test whether Arp2/3 regulated platelet actin polymerization, we used recombinant Arp2 protein (rArp2) to generate Arp2-specific antibodies (alpha Arp2). Intact and Fab fragments of alpha Arp2 inhibited TRAP-stimulated actin-polymerizing activity in platelet extracts as measured by the pyrene assay. Inhibition was reversed by the addition of rArp2 protein. To test the effect of Arp2/3 inhibition on the formation of specific actin structures, we designed a new method to permeabilize resting platelets while preserving their ability to adhere and to form filopodia and lamellipodia on exposure to glass. Inhibition of Arp2/3 froze platelets at the rounded, early stage of activation, before the formation of filopodia and lamellipodia. By morphometric analysis, the proportion of platelets in the rounded stage rose from 2.85% in untreated to 63% after treatment with alpha Arp2. This effect was also seen with Fab fragments and was reversed by the addition of rArp2 protein. By immunofluorescence of platelets at various stages of spreading, the Arp2/3 complex was found in filopodia and lamellipodia. These results suggest that activation of the Arp2/3 complex at the cortex by TRAP stimulation initiates an explosive polymerization of actin filaments that is required for all subsequent actin-dependent events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.