ArticleNucleic acids research2002
A Tetrahymena thermophila ribozyme-based indicator gene to detect transposition of marked retroelements in mammalian cells.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
- Identification of a minimal Alu domain required for retrotransposition.Nucleic acids research · 2025Article
- Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes.Nucleic acids research · 2024Article
- Article
- LINE-1 Retrotransposition Assays in Embryonic Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The L1-dependant and Pol III transcribed Alu retrotransposon, from its discovery to innate immunity.Molecular biology reports · 2021Review
- sRNA/L1 retrotransposition: using siRNAs and miRNAs to expand the applications of the cell culture-based LINE-1 retrotransposition assay.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- RNase H2, mutated in Aicardi-Goutières syndrome, promotes LINE-1 retrotransposition.The EMBO journal · 2018Article
- A Cell Fusion-Based Screening Method Identifies Glycosylphosphatidylinositol-Anchored Protein Ly6e as the Receptor for Mouse Endogenous Retroviral Envelope Syncytin-A.Journal of virology · 2017Article
- Selective elimination of long INterspersed element-1 expressing tumour cells by targeted expression of the HSV-TK suicide gene.Oncotarget · 2017Article
- Opossum APOBEC1 is a DNA mutator with retrovirus and retroelement restriction activity.Scientific reports · 2017Article
- Restricting retrotransposons: a review.Mobile DNA · 2016Review
- LINE-1 Cultured Cell Retrotransposition Assay.Methods in molecular biology (Clifton, N.J.) · 2016Article
- The Zinc-Finger Antiviral Protein ZAP Inhibits LINE and Alu Retrotransposition.PLoS genetics · 2015Article
- RNase L restricts the mobility of engineered retrotransposons in cultured human cells.Nucleic acids research · 2014Article
- Molecular reconstruction of extinct LINE-1 elements and their interaction with nonautonomous elements.Molecular biology and evolution · 2013Article
- Endogenous MOV10 inhibits the retrotransposition of endogenous retroelements but not the replication of exogenous retroviruses.Retrovirology · 2012Article
- Retrotransposition of marked SVA elements by human L1s in cultured cells.Human molecular genetics · 2011Article
- Intrinsic restriction activity by apolipoprotein B mRNA editing enzyme APOBEC1 against the mobility of autonomous retrotransposons.Nucleic acids research · 2011Article
- Structure-function analyses point to a polynucleotide-accommodating groove essential for APOBEC3A restriction activities.Journal of virology · 2011Article
- Epigenetic control of retrotransposon expression in human embryonic stem cells.Molecular and cellular biology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We devised an indicator gene for retrotransposition based on an autocatalytic ribozyme element--the Tetrahymena thermophila 23S rRNA group I intron--which can self-splice in vitro and does not require--at variance with nuclear mRNA introns--any specific pathway and cellular component for the completion of the splicing process. Several constructs, with the Tetrahymena intron adequately modified so as to be inserted at various positions within a neomycin-containing cassette under conditions that restore the neomycin-coding sequence after splicing out of the intron, were assayed for splicing efficiency in mammalian cells in culture. We show, both by northern blot analysis and by the recovery of neomycin activity upon retroviral transduction of the cassettes, that splicing efficiency depends on both the local base pairing and the global position of the intron within the neomycin transcript, and that some constructs are functional. We further show that they allow the efficient sorting out of retrotransposition events when assayed, as a control, with a human LINE retrotransposon. These indicator genes should be of great help in elucidating the mechanisms of transposition of a series of retroelements associated with transcripts not prone to nuclear mRNA intron splicing and previously not opened to any retrotransposition assay.
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Registered trials
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