ArticleNucleic acids research2002
An efficient strategy for large-scale high-throughput transposon-mediated sequencing of cDNA clones.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Genomic landscapes of bacterial transposons and their applications in strain improvement.Applied microbiology and biotechnology · 2022Review
- Extensive libraries of gene truncation variants generated by in vitro transposition.Nucleic acids research · 2017Article
- MuA-mediated in vitro cloning of circular DNA: transpositional autointegration and the effect of MuB.Molecular genetics and genomics : MGG · 2016Article
- An assay to monitor the activity of DNA transposition complexes yields a general quality control measure for transpositional recombination reactions.Mobile genetic elements · 2014Article
- Large-scale sequencing based on full-length-enriched cDNA libraries in pigs: contribution to annotation of the pig genome draft sequence.BMC genomics · 2012Article
- Insertion site preference of Mu, Tn5, and Tn7 transposons.Mobile DNA · 2012Article
- Cloning and characterization of the ravidomycin and chrysomycin biosynthetic gene clusters.Chembiochem : a European journal of chemical biology · 2010Article
- Sequencing and analysis of 10,967 full-length cDNA clones from Xenopus laevis and Xenopus tropicalis reveals post-tetraploidization transcriptome remodeling.Genome research · 2006Article
- Selection of novel vesicular stomatitis virus glycoprotein variants from a peptide insertion library for enhanced purification of retroviral and lentiviral vectors.Journal of virology · 2006Article
- Simple, robust methods for high-throughput nanoliter-scale DNA sequencing.Genome research · 2005Article
- Transposon-mediated generation of targeting vectors for the production of gene knockouts.Nucleic acids research · 2005Article
- Systematic recovery and analysis of full-ORF human cDNA clones.Genome research · 2004Article
- Chromosomal deletion formation system based on Tn5 double transposition: use for making minimal genomes and essential gene analysis.Genome research · 2003Article
- A direct transposon insertion tool for modification and functional analysis of viral genomes.Journal of virology · 2003Article
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Systematic sequencing of cDNA clones using the transposon Tn5.Nucleic acids research · 2002Article
- A Drosophila full-length cDNA resource.Genome biology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
21 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe an efficient high-throughput method for accurate DNA sequencing of entire cDNA clones. Developed as part of our involvement in the Mammalian Gene Collection full-length cDNA sequencing initiative, the method has been used and refined in our laboratory since September 2000. Amenable to large scale projects, we have used the method to generate >7 Mb of accurate sequence from 3695 candidate full-length cDNAs. Sequencing is accomplished through the insertion of Mu transposon into cDNAs, followed by sequencing reactions primed with Mu-specific sequencing primers. Transposon insertion reactions are not performed with individual cDNAs but rather on pools of up to 96 clones. This pooling strategy reduces the number of transposon insertion sequencing libraries that would otherwise be required, reducing the costs and enhancing the efficiency of the transposon library construction procedure. Sequences generated using transposon-specific sequencing primers are assembled to yield the full-length cDNA sequence, with sequence editing and other sequence finishing activities performed as required to resolve sequence ambiguities. Although analysis of the many thousands (22 785) of sequenced Mu transposon insertion events revealed a weak sequence preference for Mu insertion, we observed insertion of the Mu transposon into 1015 of the possible 1024 5mer candidate insertion sites.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.