ArticleThe Journal of cell biology2002
Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of "Christmas trees" in situ.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 136 citations in OpenAlex.
- Immunocytochemical detection of proteins within cellular structures inaccessible to specific antibodies.Histochemistry and cell biology · 2025Review
- Crossing boundaries of light microscopy resolution discerns novel assemblies in the nucleolus.Histochemistry and cell biology · 2024Article
- An evolutionarily nascent architecture underlying the formation and emergence of biomolecular condensates.Cell reports · 2023Article
- Ribosome biogenesis factors-from names to functions.The EMBO journal · 2023Review
- The axis of complement C1 and nucleolus in antinuclear autoimmunity.Frontiers in immunology · 2023Review
- Function moves biomolecular condensates in phase space.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- Review
- Splicing at the phase-separated nuclear speckle interface: a model.Nucleic acids research · 2021Review
- Recent advances in the nucleolar responses to DNA double-strand breaks.Nucleic acids research · 2020Review
- Discontinuous transcription of ribosomal DNA in human cells.PloS one · 2020Article
- Ribosomal DNA and the Nucleolus as Keystones of Nuclear Architecture, Organization, and Function.Trends in genetics : TIG · 2019Review
- Review
- Chromosome territories and the global regulation of the genome.Genes, chromosomes & cancer · 2019Review
- Review
- Cycles of protein condensation and discharge in nuclear organelles studied by fluorescence lifetime imaging.Nature communications · 2019Article
- The linker histone H1.2 is a novel component of the nucleolar organizer regions.The Journal of biological chemistry · 2018Article
- Visualization of the Nucleolus Using Ethynyl Uridine.Frontiers in plant science · 2018Article
- FGFR2 mutations in bent bone dysplasia syndrome activate nucleolar stress and perturb cell fate determination.Human molecular genetics · 2017Article
- Fluctuations of pol I and fibrillarin contents of the nucleoli.Nucleus (Austin, Tex.) · 2017Article
- Article
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
T he organization of transcriptionally active ribosomal genes in animal cell nucleoli is investigated in this study in order to address the long-standing controversy with regard to the intranucleolar localization of these genes. Detailed analyses of HeLa cell nucleoli include direct localization of ribosomal genes by in situ hybridization and their indirect localization via nascent ribosomal transcript mappings. On the light microscopy (LM) level, ribosomal genes map in 10-40 fluorescence foci per nucleus, and transcription activity is associated with most foci. We demonstrate that each nucleolar focus observed by LM corresponds, on the EM level, to an individual fibrillar center (FC) and surrounding dense fibrillar components (DFCs). The EM data identify the DFC as the nucleolar subcompartment in which rRNA synthesis takes place, consistent with detection of rDNA within the DFC. The highly sensitive method for mapping nascent transcripts in permeabilized cells on ultrastructural level provides intense and unambiguous clustered immunogold signal over the DFC, whereas very little to no label is detected over the FC. This signal is strongly indicative of nascent "Christmas trees" of rRNA associated with individual rDNA genes, sampled on the surface of thin sections. Stereological analysis of the clustered transcription signal further suggests that these Christmas trees may be contorted in space and exhibit a DNA compaction ratio on the order of 4-5.5.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.