ArticleThe Journal of general physiology2002
Absence of proton channels in COS-7 cells expressing functional NADPH oxidase components.
Article in The Journal of general physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- NADPH Oxidases (NOX): An Overview from Discovery, Molecular Mechanisms to Physiology and Pathology.Antioxidants (Basel, Switzerland) · 2021Review
- Article
- Actions and Mechanisms of Polyunsaturated Fatty Acids on Voltage-Gated Ion Channels.Frontiers in physiology · 2017Review
- The intimate and controversial relationship between voltage-gated proton channels and the phagocyte NADPH oxidase.Immunological reviews · 2016Review
- Voltage-gated proton channels: molecular biology, physiology, and pathophysiology of the H(V) family.Physiological reviews · 2013Review
- Voltage-gated proton channels.Comprehensive Physiology · 2012Review
- NOX5 in human spermatozoa: expression, function, and regulation.The Journal of biological chemistry · 2012Article
- Physiological roles of voltage-gated proton channels in leukocytes.The Journal of physiology · 2010Review
- Function of the HVCN1 proton channel in airway epithelia and a naturally occurring mutation, M91T.The Journal of general physiology · 2010Article
- Voltage-gated proton channels find their dream job managing the respiratory burst in phagocytes.Physiology (Bethesda, Md.) · 2010Review
- VSOP/Hv1 proton channels sustain calcium entry, neutrophil migration, and superoxide production by limiting cell depolarization and acidification.The Journal of experimental medicine · 2010Article
- Voltage-gated proton channels maintain pH in human neutrophils during phagocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The intimate and mysterious relationship between proton channels and NADPH oxidase.FEBS letters · 2009Article
- Detailed comparison of expressed and native voltage-gated proton channel currents.The Journal of physiology · 2008Article
- Biodiversity of voltage sensor domain proteins.Pflugers Archiv : European journal of physiology · 2007Review
- Role of nucleotides and phosphoinositides in the stability of electron and proton currents associated with the phagocytic NADPH oxidase.The Biochemical journal · 2006Article
- Article
- Electron and proton transport by NADPH oxidases.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2005Review
- Expression of gp91phox/Nox2 in COS-7 cells: cellular localization of the protein and the detection of outward proton currents.The Biochemical journal · 2005Article
- The NADPH oxidase of professional phagocytes--prototype of the NOX electron transport chain systems.Biochimica et biophysica acta · 2004Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is an enzyme of phagocytes that produces bactericidal superoxide anion (O(2)(-)) via an electrogenic process. Proton efflux compensates for the charge movement across the cell membrane. The proton channel responsible for the H(+) efflux was thought to be contained within the gp91(phox) subunit of NADPH oxidase, but recent data do not support this idea (DeCoursey, T.E., V.V. Cherny, D. Morgan, B.Z. Katz, and M.C. Dinauer. 2001. J. Biol. Chem. 276:36063-36066). In this study, we investigated electrophysiological properties and superoxide production of COS-7 cells transfected with all NADPH oxidase components required for enzyme function (COS(phox)). The 7D5 antibody, which detects an extracellular epitope of the gp91(phox) protein, labeled 96-98% of COS(phox) cells. NADPH oxidase was functional because COS(phox) (but not COS(WT)) cells stimulated by phorbol myristate acetate (PMA) or arachidonic acid (AA) produced superoxide anion. No proton currents were detected in either wild-type COS-7 cells (COS(WT)) or COS(phox) cells studied at pH(o) 7.0 and pH(i) 5.5 or 7.0. Anion currents that decayed at voltages positive to 40 mV were the only currents observed. PMA or AA did not elicit detectable H(+) current in COS(WT) or COS(phox) cells. Therefore, gp91(phox) does not function as a proton channel in unstimulated cells or in activated cells with a demonstrably functional oxidase.
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