ArticleThe Biochemical journal2002
The AtNFS2 gene from Arabidopsis thaliana encodes a NifS-like plastidial cysteine desulphurase.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 128 citations in OpenAlex.
- Identification and characterization ofHorticulture research · 2026Article
- Functional Relationships of Two NFU Proteins in Maintaining the Abundances of Mitochondrial Iron-Sulfur Proteins.Plant direct · 2025Article
- Transcriptomic analysis reveals the functions of HFrontiers in plant science · 2023Article
- Fe-S Protein Synthesis in Green Algae Mitochondria.Plants (Basel, Switzerland) · 2021Review
- Functional relationships of three NFU proteins in the biogenesis of chloroplastic iron-sulfur clusters.Plant direct · 2021Article
- Hydrogen sulfide (HaBIOTECH · 2021Review
- Iron-Sulfur Cluster Protein NITROGEN FIXATION S-LIKE1 and Its Interactor FRATAXIN Function in Plant Immunity.Plant physiology · 2020Article
- Iron-Sulfur Cluster Complex Assembly in the Mitochondria ofPlants (Basel, Switzerland) · 2020Article
- Crosstalk between Hydrogen Sulfide and Other Signal Molecules Regulates Plant Growth and Development.International journal of molecular sciences · 2020Review
- Alleviation of osmotic stress by HJournal of plant research · 2020Article
- Transcriptome profiling and identification of functional genes involved in HGenes & genomics · 2018Article
- Roles and maturation of iron-sulfur proteins in plastids.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Review
- Hydrogen Sulfide: A Gaseous Molecule in Postharvest Freshness.Frontiers in plant science · 2018Review
- Assembly and Transfer of Iron-Sulfur Clusters in the Plastid.Frontiers in plant science · 2018Review
- Silencing of the SlNAP7 gene influences plastid development and lycopene accumulation in tomato.Scientific reports · 2016Article
- Physiological Implications of Hydrogen Sulfide in Plants: Pleasant Exploration behind Its Unpleasant Odour.Oxidative medicine and cellular longevity · 2015Review
- X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Sulfur mobilization for Fe-S cluster assembly by the essential SUF pathway in the Plasmodium falciparum apicoplast and its inhibition.Antimicrobial agents and chemotherapy · 2014Article
- Article
- The suf iron-sulfur cluster synthesis pathway is required for apicoplast maintenance in malaria parasites.PLoS pathogens · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
NifS-like proteins are cysteine desulphurases required for the mobilization of sulphur from cysteine. They are present in all organisms, where they are involved in iron-sulphur (Fe-S) cluster biosynthesis. In eukaryotes, these enzymes are present in mitochondria, which are the major site for Fe-S cluster assembly. The genome of the model plant Arabidopsis thaliana contains two putative NifS-like proteins. A cDNA corresponding to one of them was cloned by reverse-transcription PCR, and named AtNFS2. The corresponding transcript is expressed in many plant tissues. It encodes a protein highly related (75% similarity) to the slr0077-gene product from Synechocystis PCC 6803, and is predicted to be targeted to plastids. Indeed, a chimaeric AtNFS2-GFP fusion protein, containing one-third of AtNFS2 from its N-terminal end, was addressed to chloroplasts. Overproduction in Escherichia coli and purification of recombinant AtNFS2 protein enabled one to demonstrate that it bears a pyridoxal 5'-phosphate-dependent cysteine desulphurase activity in vitro, thus being the first NifS homologue characterized to date in plants. The putative physiological functions of this gene are discussed, including the attractive hypothesis of a possible role in Fe-S cluster assembly in plastids.
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