ArticlePharmaceutical research2002
Quantitative analysis of correlation between number of nuclear plasmids and gene expression activity after transfection with cationic liposomes.
Article in Pharmaceutical research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 87 citations in OpenAlex.
- Mechanisms and Strategies for Enhancing DNA Nuclear Entry in Gene Delivery.Cellular and molecular bioengineering · 2026Article
- Precision enhancement of CAR-NK cells through non-viral engineering and highly multiplexed base editing.Journal for immunotherapy of cancer · 2025Article
- Copy number of naked DNA delivered into nucleus of mammalian cells by electrotransfection.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
- Review
- Article
- Lanthanide-based β-Tricalcium Phosphate Upconversion Nanoparticles as an Effective Theranostic Nonviral Vectors for Image-Guided Gene Therapy.Nanotheranostics · 2022Article
- A statistical framework for determination of minimal plasmid copy number required for transgene expression in mammalian cells.Bioelectrochemistry (Amsterdam, Netherlands) · 2021Article
- Article
- Non-viral Gene Delivery Methods for Bone and Joints.Frontiers in bioengineering and biotechnology · 2020Review
- Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection.PloS one · 2017Article
- Preclinical evaluation of an O(6)-methylguanine-DNA methyltransferase-siRNA/liposome complex administered by convection-enhanced delivery to rat and porcine brains.American journal of translational research · 2014Article
- Effect of addition of 'carrier' DNA during transient protein expression in suspension CHO culture.Cytotechnology · 2012Article
- A designed curved DNA sequence remarkably enhances transgene expression from plasmid DNA in mouse liver.Gene therapy · 2012Article
- DNA nuclear targeting sequences for non-viral gene delivery.Pharmaceutical research · 2011Article
- Nuclear entry of hyperbranched polylysine nanoparticles into cochlear cells.International journal of nanomedicine · 2011Article
- Gene therapy: a pharmacokinetic/pharmacodynamic modelling overview.Pharmaceutical research · 2010Review
- Nonviral gene delivery to mesenchymal stem cells using cationic liposomes for gene and cell therapy.Journal of biomedicine & biotechnology · 2010Article
- Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
- Efficacy of immobilized polyplexes and lipoplexes for substrate-mediated gene delivery.Biotechnology and bioengineering · 2009Article
- Nuclear delivery of NFkappaB-assisted DNA/polymer complexes: plasmid DNA quantitation by confocal laser scanning microscopy and evidence of nuclear polyplexes by FRET imaging.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeA quantitative understanding of the intracellular trafficking of plasmids delivered by nonviral vectors is essential for optimizing vector functions to increase their transfection efficiency. In this study, quantitative methods were developed to measure plasmids delivered to the nucleus, and the relationship between transfection activity and the number of plasmids in the nucleus were analyzed.
methodsAH130 cells were transfected with plasmids in cationic liposomes at various doses. The nuclear fraction was isolated after NP-40 lysis. and the unincorporated plasmids were enzvmatically degraded and washed away. Intranuclear plasmids were amplified by quantitative PCR. and the number of plasmids was determined. Plasmid amounts in the nucleus were also measured by Southern analysis to confirm the quantification.
resultsBoth methods led to similar results in measuring the nuclear plasmids within the same order of magnitude. A remarkable saturation was found for transfection activity vs. number of plasmids in the nucleus, whereas no saturation was observed in nuclear-delivered plasmids vs. dose.
conclusionsThese results clearly demonstrate the importance of the quantitative measurement of intracellular trafficking of plasmids after transfection. The findings herein described suggest that efficient transgene expression as well as enhanced nuclear delivery is required in order to achieve the maximal transfection activity of nonviral vectors.
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