ArticleMolecular pathology : MP2002
Limitations of cytokeratin 20 RT-PCR to detect disseminated tumour cells in blood and bone marrow of patients with colorectal cancer: expression in controls and downregulation in tumour tissue.
Article in Molecular pathology : MP, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 68 citations in OpenAlex.
- Relationship between vascular invasion and microvessel density and micrometastasis.World journal of gastroenterology · 2007Trial
- Common molecular markers between circulating tumor cells and blood exosomes in colorectal cancer: a systematic and analytical review.Cancer management and research · 2019Article
- The Potential for Circulating Tumor Cells in Pancreatic Cancer Management.Frontiers in physiology · 2017Review
- Molecular biomarkers for the detection of metastatic colorectal cancer cells.World journal of gastroenterology · 2014Review
- Evaluation of laparoscopic resection of colorectal carcinoma from the viewpoint of molecular biology.Wideochirurgia i inne techniki maloinwazyjne = Videosurgery and other miniinvasive techniques · 2012Article
- Multiplex RT-PCR-based detections of CEA, CK20 and EGFR in colorectal cancer patients.World journal of gastroenterology · 2010Article
- Detection of circulating tumor cells in peripheral blood from patients with gastric cancer using microRNA as a marker.Journal of molecular medicine (Berlin, Germany) · 2010Article
- Clinical relevance associated to the analysis of circulating tumour cells in patients with solid tumours.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2009Review
- Carcinoembryonic antigen and cytokeratin 20 in peritoneal cells of cancer patients: are we aware of what we are detecting by mRNA examination?British journal of cancer · 2008Article
- Detection of disseminated tumor cells in the lymph nodes of colorectal cancer patients using a real-time polymerase chain reaction assay.Langenbeck's archives of surgery · 2007Article
- Quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood of colorectal cancer patients.Journal of Zhejiang University. Science. B · 2006Article
- Molecular detection of circulating tumor cells in the peripheral blood of patients with colorectal cancer using RT-PCR: significance of the prediction of postoperative metastasis.World journal of surgery · 2006Article
- Specific detection of cytokeratin 20-positive cells in blood of colorectal and breast cancer patients by a high sensitivity real-time reverse transcriptase-polymerase chain reaction method.The Journal of molecular diagnostics : JMD · 2006Article
- Critical evaluation of real-time reverse transcriptase-polymerase chain reaction for the quantitative detection of cytokeratin 20 mRNA in colorectal cancer patients.The Journal of molecular diagnostics : JMD · 2005Article
- Combined use of positive and negative immunomagnetic isolation followed by real-time RT-PCR for detection of the circulating tumor cells in patients with colorectal cancers.Journal of molecular medicine (Berlin, Germany) · 2004Article
- Identification of occult tumor cells in node negative lymph nodes of colorectal cancer patients by cytokeratin 20 gene and protein expression.International journal of colorectal disease · 2004Article
- Association of VCAM-1 overexpression with oncogenesis, tumor angiogenesis and metastasis of gastric carcinoma.World journal of gastroenterology · 2003Article
- Nodal staging of colorectal carcinomas and sentinel nodes.Journal of clinical pathology · 2003Review
- Classification of Dukes' B and C colorectal cancers using expression arrays.Journal of cancer research and clinical oncology · 2003Article
- Detection of disseminated tumor cells in nude mice with human gastric cancer.Clinical & experimental metastasis · 2003Article
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9 authors at 1 institution in 1 country.
Funding
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Abstract
aimsDespite informative staging of patients with colorectal cancer, some patients with localised disease at diagnosis will develop recurrence or metastasis. Attempts to improve staging include sensitive detection of disseminated tumour cells in blood and bone marrow by reverse transcriptase polymerase chain reaction (RT-PCR). The results of this study have been considered in relation to the controversial results in the literature to elucidate the usefulness of cytokeratin 20 (CK20) RT-PCR to detect disseminated tumour cells further. PATIENTS/
methodsBlood and bone marrow samples from 30 patients with colorectal cancer were studied by CK20 RT-PCR. Specificity was evaluated in 47 blood and 15 bone marrow samples from non-cancer controls. In addition, the expression of CK20 mRNA and protein was studied in normal and tumour colon tissue samples.
resultsCK20 expression was detected in nine of 30 and nine of 19 of the blood and bone marrow samples from patients with colorectal cancer, respectively. In non-cancer control blood and bone marrow samples, CK20 expression was detected in 10 of 47 and four of 15, respectively. A difference between patient and control samples may be observed in terms of frequency of positive PCR tests. In tissue samples, CK20 mRNA expression was downregulated in tumour compared with normal colon tissue.
conclusionsCK20 expression was downregulated in tumour tissue compared with normal colon and a background expression of CK20 was seen in some control blood and bone marrow samples. Despite a lack of standardisation (which hampers comparison of studies), these results, together with other reports in the literature, suggest that CK20 may still be a suitable marker, but that background expression and threshold setting should be studied further.
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