ArticleThe EMBO journal2002
Fast dissociation kinetics between individual E-cadherin fragments revealed by flow chamber analysis.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 90 citations in OpenAlex.
- A Simple and Versatile Strategy for Oriented Immobilization of His-Tagged Proteins on Magnetic Nanoparticles.Bioconjugate chemistry · 2023Article
- Mathematical model of mechanobiology of acute and repeated synaptic injury and systemic biomarker kinetics.Frontiers in cellular neuroscience · 2023Article
- Integration of Cadherin Adhesion and Cytoskeleton atCold Spring Harbor perspectives in biology · 2017Review
- Review
- Possible roles of LI-Cadherin in the formation and maintenance of the intestinal epithelial barrier.Tissue barriers · 2013Review
- X-interface is not the explanation for the slow disassembly of N-cadherin dimers in the apo state.Protein science : a publication of the Protein Society · 2012Article
- Synapse adhesion: a dynamic equilibrium conferring stability and flexibility.Current opinion in neurobiology · 2012Review
- The multiparametric effects of hydrodynamic environments on stem cell culture.Tissue engineering. Part B, Reviews · 2011Review
- Dimeric states of neural- and epithelial-cadherins are distinguished by the rate of disassembly.Biochemistry · 2011Article
- Cardiac myocyte remodeling mediated by N-cadherin-dependent mechanosensing.American journal of physiology. Heart and circulatory physiology · 2011Article
- Nano-motion dynamics are determined by surface-tethered selectin mechanokinetics and bond formation.PLoS computational biology · 2009Article
- Desmocollin 3-mediated binding is crucial for keratinocyte cohesion and is impaired in pemphigus.The Journal of biological chemistry · 2009Article
- Neurexin/neuroligin interaction kinetics characterized by counting single cell-surface attached quantum dots.Biophysical journal · 2009Article
- Studying Molecular Interactions at the Single Bond Level with a Laminar Flow Chamber.Cellular and molecular bioengineering · 2008Article
- Multifunctional surfaces with discrete functionalized regions for biological applications.Langmuir : the ACS journal of surfaces and colloids · 2008Article
- The crystal structure of human E-cadherin domains 1 and 2, and comparison with other cadherins in the context of adhesion mechanism.Journal of molecular biology · 2007Article
- Dissecting subsecond cadherin bound states reveals an efficient way for cells to achieve ultrafast probing of their environment.FEBS letters · 2007Article
- Lifetime measurements reveal kinetic differences between homophilic cadherin bonds.Biophysical journal · 2006Article
- Regulation of N-cadherin dynamics at neuronal contacts by ligand binding and cytoskeletal coupling.Molecular biology of the cell · 2006Article
- E-cadherin tethered to micropatterned supported lipid bilayers as a model for cell adhesion.Langmuir : the ACS journal of surfaces and colloids · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
E-cadherin is the predominant adhesion molecule of epithelia. The interaction between extracellular segments of E-cadherin in the membrane of opposing cells is homophilic and calcium dependent. Whereas it is widely accepted that the specificity of the adhesive interaction is localized to the N-terminal domain, the kinetics of the recognition process are unknown. We report the first quantitative data describing the dissociation kinetics of individual E-cadherin interactions. Aggregation assays indicate that the two outermost domains of E-cadherin (E/EC1-2) retain biological activity when chemically immobilized on glass beads. Cadherin fragment trans-interaction was analysed using a flow chamber technique. Transient tethers had first-order kinetics, suggesting a unimolecular interaction. The unstressed lifetime of individual E-cadherin interactions was as brief as 2 s. A fast off rate and the low tensile strength of the E-cadherin bond may be necessary to support the high selectivity and plasticity of epithelial cell interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.