ArticleJournal of bacteriology2002
Membrane topology of the multidrug transporter MdfA: complementary gene fusion studies reveal a nonessential C-terminal domain.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- The Resistance-Nodulation-Division efflux pump EefABC is highly conserved within lineages ofMicrobial genomics · 2026Article
- Substrate binding in the multidrug transporter MdfA in detergent solution and in lipid nanodiscs.Biophysical journal · 2021Article
- The Multidrug Transporter MdfA Deviates from the Canonical Model of Alternating Access of MFS Transporters.Journal of molecular biology · 2020Article
- Probing the solution structure of the E. coli multidrug transporter MdfA using DEER distance measurements with nitroxide and Gd(III) spin labels.Scientific reports · 2019Article
- Biochemistry of bacterial multidrug efflux pumps.International journal of molecular sciences · 2012Review
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- A promiscuous conformational switch in the secondary multidrug transporter MdfA.The Journal of biological chemistry · 2009Article
- Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies.Molecular and cellular biochemistry · 2008Review
- No single irreplaceable acidic residues in the Escherichia coli secondary multidrug transporter MdfA.Journal of bacteriology · 2006Article
- Alkalitolerance: a biological function for a multidrug transporter in pH homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The aminoglycoside 6'-N-acetyltransferase type Ib encoded by Tn1331 is evenly distributed within the cell's cytoplasm.Antimicrobial agents and chemotherapy · 2003Article
- The Escherichia coli multidrug transporter MdfA catalyzes both electrogenic and electroneutral transport reactions.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hydrophobicity profile and sequence alignment of the Escherichia coli multidrug transporter MdfA indicate that it belongs to the 12-transmembrane-domain family of transporters. According to this prediction, MdfA contains a single membrane-embedded charged residue (Glu26), which was shown to play an important role in substrate recognition. To test the predicted secondary structure of MdfA, we analyzed complementary pairs of hybrids of MdfA-PhoA (alkaline phosphatase, functional in the periplasm) and MdfA-Cat (chloramphenicol acetyltransferase, functional in the cytoplasm), generated in all the putative cytoplasmic and periplasmic loops of MdfA. Our results support the 12-transmembrane topology model and the suggestion that except for Glu26, no other charged residues are present in the membrane domain of MdfA. Surprisingly, by testing the ability of the truncated MdfA-Cat and MdfA-PhoA hybrids to confer multidrug resistance, we demonstrate that the entire C-terminal transmembrane domain and the cytoplasmic C terminus are not essential for MdfA-mediated drug resistance and transport.
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