Evidence map›Paper›PMID 12029035›Full record

ArticleJournal of bacteriology2002

Complete nucleotide sequence of plasmid Rts1: implications for evolution of large plasmid genomes.

Takahiro Murata, Makoto Ohnishi, Takeshi Ara, Jun Kaneko, Chang-Gyun Han, Yong Fang Li, Kayoko Takashima, Hideaki Nojima, Keisuke Nakayama, Akira Kaji and 6 more

Open access · greenAbstract read
In one paragraph

Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 56 citations in OpenAlex.

  1. Article
  2. Article
  3. Development and utilization ofbioRxiv : the preprint server for biology · 2024
    Article
  4. A comprehensive list of genes required for the efficient conjugation of plasmid Rts1 was determined by systematic deletion analysis.DNA research : an international journal for rapid publication of reports on genes and genomes · 2024
    Article
  5. Review
  6. Article
  7. Article
  8. Conjugation of plasmid harboringFrontiers in microbiology · 2022
    Article
  9. Article
  10. The Global Ascendency of OXA-48-Type Carbapenemases.Clinical microbiology reviews · 2019
    Review
  11. Article
  12. Article
  13. Reconsidering plasmid maintenance factors for computational plasmid design.Computational and structural biotechnology journal · 2019
    Review
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 8 institutions in 2 countries.

Takahiro MurataDepartment of Bacteriology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
Makoto Ohnishi
Takeshi Ara
Jun Kaneko
Chang-Gyun Han
Yong Fang Li
Kayoko Takashima
Hideaki Nojima
Keisuke Nakayama
Akira Kaji
Yoshiyuki Kamio
Takeyoshi Miki
Hirotada Mori
Eiichi Ohtsubo
Yoshiro Terawaki
Tetsuya Hayashi
Keio University · JPShinshu University · JPUniversity of Miyazaki · JPThe University of Tokyo · JPTohoku University · JPKyushu University · JPNara Institute of Science and Technology · JPUniversity of Pennsylvania · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rts1, a large conjugative plasmid originally isolated from Proteus vulgaris, is a prototype for the IncT plasmids and exhibits pleiotropic thermosensitive phenotypes. Here we report the complete nucleotide sequence of Rts1. The genome is 217,182 bp in length and contains 300 potential open reading frames (ORFs). Among these, the products of 141 ORFs, including 9 previously identified genes, displayed significant sequence similarity to known proteins. The set of genes responsible for the conjugation function of Rts1 has been identified. A broad array of genes related to diverse processes of DNA metabolism were also identified. Of particular interest was the presence of tus-like genes that could be involved in replication termination. Inspection of the overall genome organization revealed that the Rts1 genome is composed of four large modules, providing an example of modular evolution of plasmid genomes.

Indexed as

Base SequenceEvolution, MolecularGenome, BacterialBacterial ProteinsChromosome MappingConjugation, GeneticDNA ReplicationDNA Transposable ElementsGenes, DuplicateMolecular Sequence DataOpen Reading FramesPlasmidsProteus vulgarisReplication OriginSequence Analysis, DNABacterial ProteinsDNA Transposable Elements

Identifiers

PMID12029035
PMCPMC135101
OpenAlexW2120490611

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.