ArticleIn vitro cellular & developmental biology. Animal2002
Generation and characterization of a conditionally immortalized lung clara cell line from the H-2Kb-tsA58 transgenic mouse.
Article in In vitro cellular & developmental biology. Animal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.
- Temperature drop and the risk of asthma: a systematic review and meta-analysis.Environmental science and pollution research international · 2017Pooled it
- Hypoxia induces adrenomedullin from lung epithelia, stimulating ILC2 inflammation and immunity.The Journal of experimental medicine · 2022Article
- Club cell-specific role of programmed cell death 5 in pulmonary fibrosis.Nature communications · 2021Article
- MOB1-YAP1/TAZ-NKX2.1 axis controls bronchioalveolar cell differentiation, adhesion and tumour formation.Oncogene · 2017Article
- C22-bronchial and T7-alveolar epithelial cell lines of the immortomouse are excellent murine cell culture model systems to study pulmonary peroxisome biology and metabolism.Histochemistry and cell biology · 2016Article
- IL-22 is produced by innate lymphoid cells and limits inflammation in allergic airway disease.PloS one · 2011Article
- Distal airways in mice exposed to cigarette smoke: Nrf2-regulated genes are increased in Clara cells.American journal of respiratory cell and molecular biology · 2008Article
- Clara cell adhesion and migration to extracellular matrix.Respiratory research · 2008Article
- Tumor necrosis factor-alpha from macrophages enhances LPS-induced clara cell expression of keratinocyte-derived chemokine.American journal of respiratory cell and molecular biology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The Clara cell is believed to be the progenitor of the peripheral airway epithelium, and it produces the surfactant proteins SP-A and SP-B, in addition to the 10-kDa Clara cell secretory protein (CCSP or CC10). To date, attempts to develop Clara cell lines have been unsuccessful. Most such attempts have involved the in vitro insertion of a transforming viral oncogene. We have reported previously the characterization of a differentiated conditionally immortalized murine lung Type II epithelial cell line, T7, from the H-2Kb-tsA58 transgenic mouse. We have also used this mouse model to derive Clara cell lines. In this model, the need for in vitro gene insertion is circumvented by the creation of a transgene, in which the large tumor antigen of a temperature-sensitive strain (tsA58) of the simian virus 40 (SV40) is fused with the major histocompatibility complex promoter H-2Kb. The promoter is active in a wide range of tissues and is induced by interferons (IFN). From the lungs of animals harboring the hybrid construct, we isolated and characterized Clara cells. The cells contain dense secretory granules and mitochondria typical of Clara cells, and express SP-A, SP-B, SP-D, and the Clara cell secretory protein, CC10. Withdrawal of the IFN and elevation of the incubation temperature permit normal cell differentiation similar to that of Clara cells in vivo. This cell line should be very useful for the investigation of normal Clara cell function and gene expression.
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