ArticleMolecular and cellular biology2002
Mitochondrial RNA import in Leishmania tropica: aptamers homologous to multiple tRNA domains that interact cooperatively or antagonistically at the inner membrane.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Exosome-Mediated Mitochondrial Delivery of Antisense Oligonucleotides.Nucleic acid therapeutics · 2025Article
- Mitochondrial genome complexity in Erodium stephanianum (Geraniaceae): nanopore sequencing reveals chloroplast gene transfer and DNA rearrangements.Frontiers in genetics · 2025Article
- Mitochondrial transport of catalytic RNAs and targeting of the organellar transcriptome in human cells.Journal of molecular cell biology · 2024Article
- Aptamer-Based Technologies for Parasite Detection.Sensors (Basel, Switzerland) · 2023Review
- Review
- Molecular and Functional Characterization of ssDNA Aptamers that Specifically Bind Leishmania infantum PABP.PloS one · 2015Article
- Small mitochondrial-targeted RNAs modulate endogenous mitochondrial protein expression in vivo.Neurobiology of disease · 2014Article
- Selection of binding targets in parasites using phage-display and aptamer libraries in vivo and in vitro.Frontiers in immunology · 2012Article
- Selection of RNA aptamers imported into yeast and human mitochondria.RNA (New York, N.Y.) · 2010Article
- Evolution of macromolecular import pathways in mitochondria, hydrogenosomes and mitosomes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
- Import of tRNAs and aminoacyl-tRNA synthetases into mitochondria.Current genetics · 2009Review
- Proton-guided movements of tRNA within the Leishmania mitochondrial RNA import complex.Nucleic acids research · 2008Article
- Necessary and sufficient factors for the import of transfer RNA into the kinetoplast mitochondrion.EMBO reports · 2007Article
- A bifunctional tRNA import receptor from Leishmania mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- In vitro selection to identify determinants in tRNA for Bacillus subtilis tyrS T box antiterminator mRNA binding.Nucleic acids research · 2005Article
- The T-stem determines the cytosolic or mitochondrial localization of trypanosomal tRNAsMet.Molecular biology of the cell · 2004Article
- A sequence motif within trypanosome precursor tRNAs influences abundance and mitochondrial localization.Molecular and cellular biology · 2003Article
- Allosteric regulation of tRNA import: interactions between tRNA domains at the inner membrane of Leishmania mitochondria.Nucleic acids research · 2003Article
- "Ping-pong" interactions between mitochondrial tRNA import receptors within a multiprotein complex.Molecular and cellular biology · 2003Article
- Transfer RNA travels from the cytoplasm to organelles.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A large number of cytoplasmic tRNAs are imported into the kinetoplast-mitochondrion of Leishmania by a receptor-mediated process. To identify the sequences recognized by import receptors, mitochondria were incubated with a combinatorial RNA library. Repeated cycles of amplification of the imported sequences (SELEX) resulted in rapid selection of several import aptamers containing sequence motifs present in the anticodon arm, the D arm, the V-T region, and acceptor stem of known tRNAs, confirming or suggesting the presence of import signals in these domains. As predicted, truncated derivatives of tRNA(Ile)(UAU) containing the D arm or the V-T region were imported in vitro. Four aptamers were studied in detail. All were imported in vitro as well as in transiently transfected cells, using the same pathway as tRNA, but their individual import efficiencies were different. Two types of aptamers were discernible: the A arm and D arm homologues (type I), which were efficiently transferred across the inner mitochondrial membrane, and the V-T homologues (type II), which were not. Remarkably, subnanomolar concentrations of type I RNAs stimulated the entry of type II RNAs into the matrix, whereas type II RNAs inhibited inner membrane transfer of type I RNAs. Moreover, tRNA(Tyr)(GUA) and tRNA(Ile)(UAU) interacted with one another as type I and type II, respectively. Such cooperative and antagonistic interactions may allow the use of a limited number of receptors to recognize a large number of tRNAs of variable affinity and enable the maintenance of a properly balanced tRNA pool for mitochondrial translation.
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