ArticleMolecular and cellular biology2002
Components of the SAGA histone acetyltransferase complex are required for repressed transcription of ARG1 in rich medium.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 61 citations in OpenAlex.
- Evolutionary diversity of the control of the azole response by Tra1 across yeast species.G3 (Bethesda, Md.) · 2024Article
- The Pseudokinase Domain ofG3 (Bethesda, Md.) · 2018Article
- Histone acetyltransferase TGF-1 regulates Trichoderma atroviride secondary metabolism and mycoparasitism.PloS one · 2018Article
- Composition of the SAGA complex in plants and its role in controlling gene expression in response to abiotic stresses.Frontiers in plant science · 2015Review
- Distinct roles of the Gcn5 histone acetyltransferase revealed during transient stress-induced reprogramming of the genome.BMC genomics · 2013Article
- A novel role for Sem1 and TREX-2 in transcription involves their impact on recruitment and H2B deubiquitylation activity of SAGA.Nucleic acids research · 2013Article
- Cellular GCN5 is a novel regulator of human adenovirus E1A-conserved region 3 transactivation.Journal of virology · 2012Article
- Chromatin and transcription in yeast.Genetics · 2012Review
- Promoter regulation by distinct mechanisms of functional interplay between lysine acetylase Rtt109 and histone chaperone Asf1.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Histone modifiers in cancer: friends or foes?Genes & cancer · 2011Article
- Distinct regulatory mechanisms of eukaryotic transcriptional activation by SAGA and TFIID.Biochimica et biophysica acta · 2011Review
- Genome-wide characterisation of the Gcn5 histone acetyltransferase in budding yeast during stress adaptation reveals evolutionarily conserved and diverged roles.BMC genomics · 2010Article
- Histone modifying proteins Gcn5 and Hda1 affect flocculation in Saccharomyces cerevisiae during high-gravity fermentation.Current genetics · 2010Article
- The SAGA subunit Ada2 functions in transcriptional silencing.Molecular and cellular biology · 2009Article
- The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8.Genes & development · 2008Article
- Nucleosome positioning and histone H3 acetylation are independent processes in the Aspergillus nidulans prnD-prnB bidirectional promoter.Eukaryotic cell · 2008Article
- Structure/function analysis of the phosphatidylinositol-3-kinase domain of yeast tra1.Genetics · 2007Article
- The SAGA continues: expanding the cellular role of a transcriptional co-activator complex.Oncogene · 2007Review
- C-terminal processing of yeast Spt7 occurs in the absence of functional SAGA complex.BMC biochemistry · 2007Article
- Isolation and characterization of a GCN5-interacting protein from Arabidopsis thaliana.Planta · 2007Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptional regulation of the Saccharomyces cerevisiae ARG1 gene is controlled by positive and negative elements. The transactivator Gcn4p is required for activation in minimal medium, while arginine repression requires the ArgR/Mcm1 regulatory complex, which binds to two upstream arginine control elements. We have found that the coordinated regulation of ARG1 requires components of the SAGA chromatin-remodeling complex. Using gcn5 deletion strains and a Gcn5 protein carrying the E173Q mutation in the histone acetyltransferase (HAT) region, we show that the HAT activity of Gcn5p is required for repression of ARG1 in rich medium. Similar increases in expression were seen upon deletion of other SAGA components but not upon deletion of the ADA-specific component, Ahc1p. Chromatin immunoprecipitations using antibodies to acetylated H3 confirmed that a decrease in the level of acetylated histones at the ARG1 promoter correlated with increased ARG1 expression. Up-regulation of ARG1 in the absence of Gcn5p also correlated with increased binding of TATA-binding protein to the promoter. The analysis of promoter deletions showed that Gcn5/Ada repression of ARG1 was mediated through the action of the ArgR/Mcm1 regulatory complex. In addition, studies with minimal medium demonstrated a requirement for the Ada proteins in activation of ARG1. This suggests that SAGA has a dual role at ARG1, acting to repress transcription in rich medium and activate transcription in minimal medium.
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