ArticleMolecular and cellular biology2002
The E2 ubiquitin conjugase Rad6 is required for the ArgR/Mcm1 repression of ARG1 transcription.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Histone H2B ubiquitylation: Connections to transcription and effects on chromatin structure.Biochimica et biophysica acta. Gene regulatory mechanisms · 2024Review
- Potent macrocycle inhibitors of the human SAGA deubiquitinating module.Cell chemical biology · 2022Article
- Expanding Role of Ubiquitin in Translational Control.International journal of molecular sciences · 2020Review
- Chemo-Genetic Interactions Between Histone Modification and the Antiproliferation Drug AICAR Are Conserved in Yeast and Humans.Genetics · 2016Article
- Genomic instability in human cancer: Molecular insights and opportunities for therapeutic attack and prevention through diet and nutrition.Seminars in cancer biology · 2015Review
- The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination.The Journal of biological chemistry · 2014Article
- Effects of the Paf1 complex and histone modifications on snoRNA 3'-end formation reveal broad and locus-specific regulation.Molecular and cellular biology · 2013Article
- Shaping the landscape: mechanistic consequences of ubiquitin modification of chromatin.EMBO reports · 2012Review
- Paf1 restricts Gcn4 occupancy and antisense transcription at the ARG1 promoter.Molecular and cellular biology · 2012Article
- Article
- The Paf1 complex represses ARG1 transcription in Saccharomyces cerevisiae by promoting histone modifications.Eukaryotic cell · 2011Article
- Histone H2B ubiquitination and beyond: Regulation of nucleosome stability, chromatin dynamics and the trans-histone H3 methylation.Epigenetics · 2010Review
- Ubiquitination of histone H2B regulates chromatin dynamics by enhancing nucleosome stability.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The cap binding complex influences H2B ubiquitination by facilitating splicing of the SUS1 pre-mRNA.RNA (New York, N.Y.) · 2009Article
- Chromatin structure and DNA damage repair.Epigenetics & chromatin · 2008Article
- The role of histone ubiquitylation and deubiquitylation in gene expression as determined by the analysis of an HTB1(K123R) Saccharomyces cerevisiae strain.Molecular genetics and genomics : MGG · 2007Article
- Dot1a-AF9 complex mediates histone H3 Lys-79 hypermethylation and repression of ENaCalpha in an aldosterone-sensitive manner.The Journal of biological chemistry · 2006Article
- The deubiquitylation activity of Ubp8 is dependent upon Sgf11 and its association with the SAGA complex.Molecular and cellular biology · 2005Article
- Effectors of lysine 4 methylation of histone H3 in Saccharomyces cerevisiae are negative regulators of PHO5 and GAL1-10.The Journal of biological chemistry · 2004Article
- Rad6-Bre1-mediated histone H2B ubiquitylation modulates the formation of double-strand breaks during meiosis.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription of the Saccharomyces cerevisiae ARG1 gene is under the control of both positive and negative elements. Activation of the gene in minimal medium is induced by Gcn4. Repression occurs in the presence of arginine and requires the ArgR/Mcm1 complex that binds to two upstream arginine control (ARC) elements. With the recent finding that the E2 ubiquitin conjugase Rad6 modifies histone H2B, we examined the role of Rad6 in the regulation of ARG1 transcription. We find that Rad6 is required for repression of ARG1 in rich medium, with expression increased approximately 10-fold in a rad6 null background. Chromatin immunoprecipitation analysis indicates increased binding of TATA-binding protein in the absence of Rad6. The active-site cysteine of Rad6 is required for repression, implicating ubiquitination in the process. The effects of Rad6 at ARG1 involve two components. In one of these, histone H2B is the likely target for ubiquitination by Rad6, since a strain expressing histone H2B with the principal ubiquitination site converted from lysine to arginine shows a fivefold relief of repression. The second component requires Ubr1 and thus likely the pathway of N-end rule degradation. Through the analysis of promoter constructs with ARC deleted and an arg80 rad6 double mutant, we show that Rad6 repression is mediated through the ArgR/Mcm1 complex. In addition, analysis of an ada2 rad6 deletion strain indicated that the SAGA acetyltransferase complex and Rad6 act in the same pathway to repress ARG1 in rich medium.
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