ArticleThe Biochemical journal2002
Activation of mouse Pi-class glutathione S-transferase gene by Nrf2(NF-E2-related factor 2) and androgen.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 91 citations in OpenAlex.
- The Ancestral KEAP1-NRF Pathway in AmphioxusInternational journal of molecular sciences · 2025Article
- Cisplatin-based Liposomal Nanocarriers for Drug Delivery in Lung Cancer Therapy: Recent Progress and Future Outlooks.Current pharmaceutical design · 2024Review
- The good Samaritan glutathione-S-transferase P1: An evolving relationship in nitric oxide metabolism mediated by the direct interactions between multiple effector molecules.Redox biology · 2023Review
- Targeting GSTP1-dependent ferroptosis in lung cancer radiotherapy: Existing evidence and future directions.Frontiers in molecular biosciences · 2022Review
- Developmental adverse effects of trace amounts of lead: Evaluation using zebrafish model.Frontiers in pharmacology · 2022Article
- Sensitization of A-549 lung cancer cells to Cisplatin by Quinacrine-loaded lipidic nanoparticles via suppressing Nrf2 mediated defense mechanism.Naunyn-Schmiedeberg's archives of pharmacology · 2021Article
- Liver-specific knockout of histone methyltransferase G9a impairs liver maturation and dysregulates inflammatory, cytoprotective, and drug-processing genes.Xenobiotica; the fate of foreign compounds in biological systems · 2019Article
- Antioxidant response elements: Discovery, classes, regulation and potential applications.Redox biology · 2018Review
- Article
- Dual NRF2 paralogs in Coho salmon and their antioxidant response element targets.Redox biology · 2016Article
- A novel crosstalk between the tumor suppressors ING1 and ING2 regulates androgen receptor signaling.Journal of molecular medicine (Berlin, Germany) · 2016Article
- One-Carbon Metabolism in Prostate Cancer: The Role of Androgen Signaling.International journal of molecular sciences · 2016Review
- Downregulation of Reactive Oxygen Species in Apoptosis.Journal of cancer prevention · 2016Review
- Antioxidant Rescue of Selenomethionine-Induced Teratogenesis in Zebrafish Embryos.Archives of environmental contamination and toxicology · 2016Article
- Identification of a functional antioxidant response element within the eighth intron of the human ABCC3 gene.Drug metabolism and disposition: the biological fate of chemicals · 2015Article
- In vivo regulation of human glutathione transferase GSTP by chemopreventive agents.Cancer research · 2014Article
- The bromodomain protein BRD4 regulates the KEAP1/NRF2-dependent oxidative stress response.Cell death & disease · 2014Article
- Piper betle induces phase I & II genes through Nrf2/ARE signaling pathway in mouse embryonic fibroblasts derived from wild type and Nrf2 knockout cells.BMC complementary and alternative medicine · 2014Article
- Regulation of SIRT1 by oxidative stress-responsive miRNAs and a systematic approach to identify its role in the endothelium.Antioxidants & redox signaling · 2013Review
- Zinc and the modulation of redox homeostasis.Free radical biology & medicine · 2012Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Pi-class glutathione S-transferases (GSTs) play pivotal roles in the detoxification of xenobiotics, carcinogenesis and drug resistance. The mechanisms of regulation of these genes during drug induction and carcinogenesis are yet to be elucidated. Recently, Nrf2 (NF-E2-related factor 2; a bZip-type transcription factor) knockout mice were shown to display impaired induction of Pi-class GST genes by drugs. It is known that the mouse Pi-class GST gene GST-P1 is expressed predominantly in the male liver, and is regulated by androgen. To determine whether Nrf2 and the androgen receptor regulate GST-P1 directly, we analysed the molecular mechanism of activation of this gene by these factors. The promoter of the GST-P1 gene was activated markedly by Nrf2 in transient transfection analyses. Gel mobility shift assay and footprinting analyses revealed three Nrf2 binding sites: one at the proximal and two at distal elements, located at positions -59, -915 and -937 from the cap site. The fifth intron of the GST-P1 gene contains the androgen-responsive region. Multiple androgen receptor binding sites are clustered within a 500 bp region of this intron. The whole fragment contains a minimum of seven androgen receptor binding sites, which collectively display strong androgen-dependent enhancer activity. However, on division into small fragments containing two or three elements each, individual enhancer activities were dramatically decreased. This suggests that multiple elements work synergistically as a strong androgen-responsive enhancer. Our findings indicate that Nrf2 and the androgen receptor directly bind to and activate the mouse GST-P1 gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.