ArticleThe Biochemical journal2002
Purification of the Escherichia coli ammonium transporter AmtB reveals a trimeric stoichiometry.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 95 citations in OpenAlex.
- Bacterial sensing and response for neutralization and detoxification of environmental ammonia.Journal of bacteriology · 2026Article
- Flipping the switch: dynamic modulation of membrane transporter activity in bacteria.Microbiology (Reading, England) · 2023Review
- Prokaryotic ammonium transporters: what has three decades of research revealed?Microbiology (Reading, England) · 2023Article
- Exaggerated trans-membrane charge of ammonium transporters in nutrient-poor marine environments.Open biology · 2022Article
- COVID-19 coagulopathies: Human blood proteins mimic SARS-CoV-2 virus, vaccine proteins and bacterial co-infections inducing autoimmunity: Combinations of bacteria and SARS-CoV-2 synergize to induce autoantibodies targeting cardiolipin, cardiolipin-binding proteins, platelet factor 4, prothrombin, and coagulation factors.BioEssays : news and reviews in molecular, cellular and developmental biology · 2021Article
- Function and Regulation of Ammonium Transporters in Plants.International journal of molecular sciences · 2020Review
- The lipid environment determines the activity of the Escherichia coli ammonium transporter AmtB.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- The ctenidium of the giant clam, Tridacna squamosa, expresses an ammonium transporter 1 that displays light-suppressed gene and protein expression and may be involved in ammonia excretion.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2018Article
- The Kinase CIPK23 Inhibits Ammonium Transport inThe Plant cell · 2017Article
- How Do Short Chain Nonionic Detergents Destabilize G-Protein-Coupled Receptors?Journal of the American Chemical Society · 2016Article
- Physical and genetic interaction between ammonium transporters and the signaling protein Rho1 in the plant pathogen Ustilago maydis.Eukaryotic cell · 2014Article
- Article
- Nitrogen assimilation in Escherichia coli: putting molecular data into a systems perspective.Microbiology and molecular biology reviews : MMBR · 2013Review
- The molecular basis of K+ exclusion by the Escherichia coli ammonium channel AmtB.The Journal of biological chemistry · 2013Article
- High-throughput genome sequencing of lichenizing fungi to assess gene loss in the ammonium transporter/ammonia permease gene family.BMC genomics · 2013Article
- Periplasmic vestibule plays an important role for solute recruitment, selectivity, and gating in the Rh/Amt/MEP superfamily.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Epistatic effects of the protease/chaperone HflB on some damaged forms of the Escherichia coli ammonium channel AmtB.Genetics · 2009Article
- Pore mutations in ammonium transporter AMT1 with increased electrogenic ammonium transport activity.The Journal of biological chemistry · 2009Article
- Detergent binding explains anomalous SDS-PAGE migration of membrane proteins.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Heterologous expression of a membrane-spanning auxin importer: implications for functional analyses of auxin transporters.International journal of plant genomics · 2009Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Amt family of high-affinity ammonium transporters is a family of integral membrane proteins that are found in archaea, bacteria, fungi, plants and animals. Furthermore, the family has recently been extended to humans with the recognition that both the erythroid and non-erythroid Rhesus proteins are also ammonium transporters. The Escherichia coli AmtB protein offers a good model system for the Amt family and in order to address questions relating to both its structure and function we have overproduced a histidine-tagged form of the protein (AmtB6H) and purified it to homogeneity. We examined the quaternary structure of AmtB6H (which is active in vivo) by SDS/PAGE, gel-filtration chromatography, dynamic light scattering and sedimentation ultracentrifugation. The protein was resistant to dissociation by SDS and behaved as a stable oligomer on SDS/PAGE. By equilibrium desorption chromatography we determined the mass ratio of dodecyl beta-D-maltoside to AmtB in the detergent-solubilized complex to be 1.03+/-0.03, and this allowed us to calculate, from analytical-ultracentrifugation data, that AmtB purifies as a trimer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.