ArticleThe Biochemical journal2002
Retinoic acid receptor-related orphan receptor (ROR) alpha4 is the predominant isoform of the nuclear receptor RORalpha in the liver and is up-regulated by hypoxia in HepG2 human hepatoma cells.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- Importance of STAT3 signaling in preeclampsia (Review).International journal of molecular medicine · 2025Review
- Induction of hepatic fibrosis in mice with schistosomiasis by extracellular microRNA-30 derived fromFrontiers in immunology · 2024Article
- RAR-related orphan receptor alpha and the staggerer mice: a fine molecular story.Frontiers in endocrinology · 2023Review
- ROS inhibits RORα degradation by decreasing its arginine methylation in liver cancer.Cancer science · 2023Article
- RAR-Related Orphan Receptor: An Accelerated Preeclampsia Progression by Activating the JAK/STAT3 Pathway.Yonsei medical journal · 2022Article
- On the relationship between VDR, RORα and RORγ receptors expression and HIF1-α levels in human melanomas.Experimental dermatology · 2019Article
- Retinoic acid receptor-related orphan receptor RORα regulates differentiation and survival of keratinocytes during hypoxia.Journal of cellular physiology · 2018Article
- Retinoic Acid Receptor-Related Orphan Receptors: Critical Roles in Tumorigenesis.Frontiers in immunology · 2018Review
- RORα2 requires LSD1 to enhance tumor progression in breast cancer.Scientific reports · 2017Article
- RORC2 Genetic Variants and Serum Levels in Patients with Rheumatoid Arthritis.International journal of molecular sciences · 2016Article
- RORα, a potential tumor suppressor and therapeutic target of breast cancer.International journal of molecular sciences · 2012Article
- Regulation of p53 stability and apoptosis by a ROR agonist.PloS one · 2012Article
- Cell-autonomous and non-cell-autonomous neuroprotective functions of RORα in neurons and astrocytes during hypoxia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Convergence of linkage, gene expression and association data demonstrates the influence of the RAR-related orphan receptor alpha (RORA) gene on neovascular AMD: a systems biology based approach.Vision research · 2010Article
- The nuclear receptor ROR(alpha) exerts a bi-directional regulation of IL-6 in resting and reactive astrocytes.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Identification of human CYP2C8 as a retinoid-related orphan nuclear receptor target gene.The Journal of pharmacology and experimental therapeutics · 2009Article
- Retinoid-related orphan receptors (RORs): critical roles in development, immunity, circadian rhythm, and cellular metabolism.Nuclear receptor signaling · 2009Review
- Article
- Retinoid-related Orphan Receptors (RORs): Roles in Cellular Differentiation and Development.Advances in developmental biology (Amsterdam, Netherlands) · 2006Article
- The gene encoding human retinoic acid-receptor-related orphan receptor alpha is a target for hypoxia-inducible factor 1.The Biochemical journal · 2004Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The retinoic acid receptor-related orphan receptor alpha (RORalpha) is critically involved in many physiological functions in several organs. We find that the main RORalpha isoform in the mouse liver is the RORalpha4 isoform, in terms of both mRNA and protein levels, while the RORalpha1 isoform is less abundant. Because hypoxia is a major feature of liver physiology and pathology, we examined the effect of this stress on Rora gene expression and RORalpha transcriptional activity. HepG2 human hepatoma cells were cultured for 24 h under normoxia (20% O2) or hypoxia (10, 2, and 0.1% O2) and the abundance of the Rora transcripts measured by Northern blot and semi-quantitative RT-PCR. Hypoxic HepG2 cells contained more Rora mRNA than controls. This was also observed in rat hepatocytes in primary culture. Cobalt chloride and desferrioxamine also increased the amount of Rora mRNA in HepG2 cells. It is likely that these treatments increase the amount of the RORalpha4 protein in HepG2 cells as evidenced by Western blotting in the case of desferrioxamine. Transient transfection experiments indicated that hypoxia, cobalt chloride, and desferrioxamine all stimulate RORalpha transcriptional activity in HepG2 cells. Hence, we believe that RORalpha participates in the control of gene transcription in hepatic cells and modulates gene expression in response to hypoxic stress.
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