ArticleGenes & development2002
Xic1 degradation in Xenopus egg extracts is coupled to initiation of DNA replication.
Article in Genes & development, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 41 citations in OpenAlex.
- Acute Oxidative Effect and Muscle Damage after a Maximum 4 Min Test in High Performance Athletes.PloS one · 2016Article
- MYC and the control of DNA replication.Cold Spring Harbor perspectives in medicine · 2014Review
- Direct role for proliferating cell nuclear antigen in substrate recognition by the E3 ubiquitin ligase CRL4Cdt2.The Journal of biological chemistry · 2012Article
- Mechanism of CRL4(Cdt2), a PCNA-dependent E3 ubiquitin ligase.Genes & development · 2011Review
- Molecular architecture of the DNA replication origin activation checkpoint.The EMBO journal · 2010Article
- Docking of a specialized PIP Box onto chromatin-bound PCNA creates a degron for the ubiquitin ligase CRL4Cdt2.Molecular cell · 2009Article
- The Xenopus cell cycle: an overview.Molecular biotechnology · 2008Review
- ATM activation and its recruitment to damaged DNA require binding to the C terminus of Nbs1.Molecular and cellular biology · 2005Article
- Replication-dependent destruction of Cdt1 limits DNA replication to a single round per cell cycle in Xenopus egg extracts.Genes & development · 2005Article
- Role for Cdk1 (Cdc2)/cyclin A in preventing the mammalian origin recognition complex's largest subunit (Orc1) from binding to chromatin during mitosis.Molecular and cellular biology · 2004Article
- The role of Cdc6 in ensuring complete genome licensing and S phase checkpoint activation.The Journal of cell biology · 2004Article
- Assembly of the replication initiation complex on SV40 origin DNA.Nucleic acids research · 2004Article
- Xkid is degraded in a D-box, KEN-box, and A-box-independent pathway.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
CDK2 activity is regulated by phosphorylation/dephosphorylation, subcellular localization, cyclin levels, and cyclin dependent kinase inhibitors (CKIs). Using Xenopus egg extracts, we find that degradation of Xic1, a Xenopus p21(cip1)/p27(kip1) family member, is coupled to initiation of DNA replication. Xic1 turnover requires the formation of a prereplication complex (pre-RC). Additionally, downstream initiation factors including CDK2, Cdc7, and Cdc45, but not RPA or DNA polymerase alpha, are necessary for activating the degradation system. Xic1 degradation is attenuated following completion of DNA replication. Unlike degradation of p27(kip1) in mammalian cells, CDK2 activity is not directly involved in Xic1 degradation and interactions between Xic1 and CDK2/cyclin E are dispensable for Xic1 turnover. Interestingly, a C-terminal region (162-192) of Xic1 is essential and apparently sufficient for triggering Xic1 ubiquitination prior to degradation. These observations demonstrate that a direct link exists between DNA replication and CKI degradation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.