ArticleBiophysical journal2002
Dissecting streptavidin-biotin interaction with a laminar flow chamber.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
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- Is There a Need for a More Precise Description of Biomolecule Interactions to Understand Cell Function?Current issues in molecular biology · 2022Review
- Mechanotransduction as a major driver of cell behaviour: mechanisms, and relevance to cell organization and future research.Open biology · 2021Review
- Engineering a disulfide-gated switch in streptavidin enables reversible binding without sacrificing binding affinity.Scientific reports · 2020Article
- Biotin-Avidin-Mediated Capture of Microspheres on Polymer Fibers.Molecules (Basel, Switzerland) · 2019Article
- Nanobody-CD16 Catch Bond Reveals NK Cell Mechanosensitivity.Biophysical journal · 2019Article
- Dual Recognition Element Lateral Flow Assay Toward Multiplex Strain Specific Influenza Virus Detection.Analytical chemistry · 2017Article
- Bead mediated separation of microparticles in droplets.PloS one · 2017Article
- A Rough Energy Landscape to Describe Surface-Linked Antibody and Antigen Bond Formation.Scientific reports · 2016Article
- Magnetic fingerprints of rolling cells for quantitative flow cytometry in whole blood.Scientific reports · 2016Article
- The cellular environment regulates in situ kinetics of T-cell receptor interaction with peptide major histocompatibility complex.European journal of immunology · 2015Article
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- Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of Lactococcus lactis.PloS one · 2013Article
- Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.ACS nano · 2012Article
- In vivo PEG modification of vascular surfaces for targeted delivery.Journal of vascular surgery · 2012Article
- Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Kinetics and mechanics of two-dimensional interactions between T cell receptors and different activating ligands.Biophysical journal · 2012Article
- Article
- How the biotin-streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer.The Biochemical journal · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A laminar flow chamber was used to study single molecule interactions between biotinylated surfaces and streptavidin-coated spheres subjected to a hydrodynamic drag lower than a piconewton. Spheres were tracked with 20 ms and 40 nm resolution. They displayed multiple arrests lasting between a few tens of milliseconds and several minutes or more. Analysis of about 500,000 positions revealed that streptavidin-biotin interaction was multiphasic: transient bound states displayed a rupture frequency of 5.3 s(-1) and a rate of transition toward a more stable configuration of 1.3 s(-1). These parameters did not display any significant change when the force exerted on bonds varied between 3.5 and 11 pN. However, the apparent rate of streptavidin-biotin association exhibited about 10-fold decrease when the wall shear rate was increased from 7 to 22 s(-1), which supports the existence of an energy barrier opposing the formation of the transient binding state. It is concluded that a laminar flow chamber can yield new and useful information on the formation of molecular bonds, and especially on the structure of the external part of the energy landscape of ligand-receptor complexes.
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