ArticleBiophysical journal2002
Whole cell patch clamp recording performed on a planar glass chip.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
62 citing papers in PubMed, 347 citations in OpenAlex.
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- Synthesis of Orthorhombic Tin Dioxide Nanowires in Track Templates.Materials (Basel, Switzerland) · 2024Article
- A Comparative Study on the Lysosomal Cation Channel TMEM175 Using Automated Whole-Cell Patch-Clamp, Lysosomal Patch-Clamp, and Solid Supported Membrane-Based Electrophysiology: Functional Characterization and High-Throughput Screening Assay Development.International journal of molecular sciences · 2023Article
- High-Efficiency Single-Cell Electrical Impedance Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- An Overview of In Vitro Biological Neural Networks for Robot Intelligence.Cyborg and bionic systems (Washington, D.C.) · 2023Review
- Development of automated patch clamp assays to overcome the burden of variants of uncertain significance in inheritable arrhythmia syndromes.Frontiers in physiology · 2023Review
- Systematic and mechanistic analysis of AuNP-induced nanotoxicity for risk assessment of nanomedicine.Nano convergence · 2022Review
- Highly Parallelized, Multicolor Optogenetic Recordings of Cellular Activity for Therapeutic Discovery Applications in Ion Channels and Disease-Associated Excitable Cells.Frontiers in molecular neuroscience · 2022Article
- Measuring Absolute Membrane Potential Across Space and Time.Annual review of biophysics · 2021Review
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- TbScience advances · 2021Article
- VoltageFluor dyes and fluorescence lifetime imaging for optical measurement of membrane potential.Methods in enzymology · 2021Article
- A Temperature-Controlled Patch Clamp Platform Demonstrated on Jurkat T Lymphocytes and Human Induced Pluripotent Stem Cell-Derived Neurons.Bioengineering (Basel, Switzerland) · 2020Article
- A nanoelectrode array for obtaining intracellular recordings from thousands of connected neurons.Nature biomedical engineering · 2020Article
- PatcherBot: a single-cell electrophysiology robot for adherent cells and brain slices.Journal of neural engineering · 2019Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The state of the art technology for the study of ion channels is the patch clamp technique. Ion channels mediate electrical current flow, have crucial roles in cellular physiology, and are important drug targets. The most popular (whole cell) variant of the technique detects the ensemble current over the entire cell membrane. Patch clamping is still a laborious process, requiring a skilled experimenter to micromanipulate a glass pipette under a microscope to record from one cell at a time. Here we report on a planar, microstructured quartz chip for whole cell patch clamp measurements without micromanipulation or visual control. A quartz substrate of 200 microm thickness is perforated by wet etching techniques resulting in apertures with diameters of approximately 1 microm. The apertures replace the tip of glass pipettes commonly used for patch clamp recording. Cells are positioned onto the apertures from suspension by application of suction. Whole cell recordings from different cell types (CHO, N1E-115 neuroblastoma) are performed with microstructured chips studying K(+) channels and voltage gated Ca(2+) channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.