ArticleBiophysical journal2002
Single cell mechanotransduction and its modulation analyzed by atomic force microscope indentation.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed.
- Insights into the alteration of osteoblast mechanical properties upon adhesion on chitosan.BioMed research international · 2014Trial
- Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential.Advanced healthcare materials · 2026Review
- The matrix stiffness is increased in the eutopic endometrium of adenomyosis patients: a study based on atomic force microscopy and histochemistry.European journal of histochemistry : EJH · 2024Article
- Probing living cell dynamics and molecular interactions using atomic force microscopy.Biophysical reviews · 2024Review
- Magnetically Driven Hydrogel Surfaces for Modulating Macrophage Behavior.ACS biomaterials science & engineering · 2024Article
- Purinergic signaling through the P2Y2 receptor regulates osteocytes' mechanosensitivity.The Journal of cell biology · 2024Article
- Applying the Atomic Force Microscopy Technique in Medical Sciences-A Narrative Review.Biomedicines · 2024Review
- Ligand Binding-Induced Cellular Membrane Deformation is Correlated with the Changes in Membrane Stiffness.The journal of physical chemistry. B · 2023Article
- From cells to form: A roadmap to study shape emergence in vivo.Biophysical journal · 2023Review
- The effects of mechanical force on fibroblast behavior in cutaneous injury.Frontiers in surgery · 2023Review
- Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy.Progress in biophysics and molecular biology · 2022Review
- Unbiased retrieval of frequency-dependent mechanical properties from noisy time-dependent signals.Biophysical reports · 2022Article
- The role of cellular traction forces in deciphering nuclear mechanics.Biomaterials research · 2022Review
- Vascular Endothelial Growth Factor Receptor-1 Modulates Hypoxia-Mediated Endothelial Senescence and Cellular Membrane StiffnessFrontiers in cell and developmental biology · 2022Article
- Simulating microgravity using a random positioning machine for inducing cellular responses to mechanotransduction in human osteoblasts.The Review of scientific instruments · 2021Article
- Engineered Extracellular Matrices with Integrated Wireless Microactuators to Study Mechanobiology.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Mechanobiological evaluation of prostate cancer metastasis to bone using an in vitro prostate cancer testbed.Journal of biomechanics · 2021Article
- Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes.Micromachines · 2020Article
- A Comprehensive Review on the Synthesis, Characterization, and Biomedical Application of Platinum Nanoparticles.Nanomaterials (Basel, Switzerland) · 2019Review
- Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM-FRAP.Small (Weinheim an der Bergstrasse, Germany) · 2019Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The skeleton adapts to its mechanical usage, although at the cellular level, the distribution and magnitude of strains generated and their detection are ill-understood. The magnitude and nature of the strains to which cells respond were investigated using an atomic force microscope (AFM) as a microindentor. A confocal microscope linked to the setup enabled analysis of cellular responses. Two different cell response pathways were identified: one, consequent upon contact, depended on activation of stretch-activated ion channels; the second, following stress relaxation, required an intact microtubular cytoskeleton. The cellular responses could be modulated by selectively disrupting cytoskeletal components thought to be involved in the transduction of mechanical stimuli. The F-actin cytoskeleton was not required for responses to mechanical strain, whereas the microtubular and vimentin networks were. Treatments that reduced membrane tension, or its transmission, selectively reduced contact reactions. Immunostaining of the cell cytoskeleton was used to interpret the results of the cytoskeletal disruption studies. We provide an estimate of the cellular strain magnitude needed to elicit intracellular calcium responses and propose a model that links single cell responses to whole bone adaptation. This technique may help to understand adaptation to mechanical usage in other organs.
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