ArticleBMC microbiology2002
The methionine salvage pathway in Bacillus subtilis.
Article in BMC microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 97 citations in OpenAlex.
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- Revisiting the methionine salvage pathway and its paralogues.Microbial biotechnology · 2019Article
- A Survey of Pyridoxal 5'-Phosphate-Dependent Proteins in the Gram-Positive Model BacteriumFrontiers in molecular biosciences · 2019Review
- Activation of a gene network in durum wheat roots exposed to cadmium.BMC plant biology · 2018Article
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- Spermidine promotesThe Journal of biological chemistry · 2017Article
- Flavoenzyme CrmK-mediated substrate recycling in caerulomycin biosynthesis.Chemical science · 2016Article
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- Integration of untargeted metabolomics with transcriptomics reveals active metabolic pathways.Metabolomics : Official journal of the Metabolomic Society · 2014Article
- Hierarchical expression of genes controlled by the Bacillus subtilis global regulatory protein CodY.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- MtnBD is a multifunctional fusion enzyme in the methionine salvage pathway of Tetrahymena thermophila.PloS one · 2013Article
- Enzymatic characterization of AMP phosphorylase and ribose-1,5-bisphosphate isomerase functioning in an archaeal AMP metabolic pathway.Journal of bacteriology · 2012Article
- Mechanistic diversity in the RuBisCO superfamily: RuBisCO from Rhodospirillum rubrum is not promiscuous for reactions catalyzed by RuBisCO-like proteins.Biochemistry · 2012Article
- A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis.Nature chemical biology · 2012Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPolyamine synthesis produces methylthioadenosine, which has to be disposed of. The cell recycles it into methionine through methylthioribose (MTR). Very little was known about MTR recycling for methionine salvage in Bacillus subtilis.
resultsUsing in silico genome analysis and transposon mutagenesis in B. subtilis we have experimentally uncovered the major steps of the dioxygen-dependent methionine salvage pathway, which, although similar to that found in Klebsiella pneumoniae, recruited for its implementation some entirely different proteins. The promoters of the genes have been identified by primer extension, and gene expression was analyzed by Northern blotting and lacZ reporter gene expression. Among the most remarkable discoveries in this pathway is the role of an analog of ribulose diphosphate carboxylase (Rubisco, the plant enzyme used in the Calvin cycle which recovers carbon dioxide from the atmosphere) as a major step in MTR recycling.
conclusionsA complete methionine salvage pathway exists in B. subtilis. This pathway is chemically similar to that in K. pneumoniae, but recruited different proteins to this purpose. In particular, a paralogue or Rubisco, MtnW, is used at one of the steps in the pathway. A major observation is that in the absence of MtnW, MTR becomes extremely toxic to the cell, opening an unexpected target for new antimicrobial drugs. In addition to methionine salvage, this pathway protects B. subtilis against dioxygen produced by its natural biotope, the surface of leaves (phylloplane).
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