ArticleRNA (New York, N.Y.)2002
In vivo selection of better self-splicing introns in Escherichia coli: the role of the P1 extension helix of the Tetrahymena intron.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Efficient circularization of protein-encoding RNAs via a novel cis-splicing system.Nucleic acids research · 2024Article
- Development of advanced control material for reverse transcription-mediated bacterial nucleic acid amplification tests.Journal of clinical microbiology · 2024Article
- Efficient circular RNA engineering by end-to-end self-targeting and splicing reaction usingMolecular therapy. Nucleic acids · 2023Article
- Targeted editing and evolution of engineered ribosomes in vivo by filtered editing.Nature communications · 2022Article
- Intron-assisted, viroid-based production of insecticidal circular double-stranded RNA inRNA biology · 2021Article
- Fitness landscape of a dynamic RNA structure.PLoS genetics · 2021Article
- Prebiotic RNA Network Formation: A Taxonomy of Molecular Cooperation.Life (Basel, Switzerland) · 2017Article
- Design and Experimental Evolution of trans-Splicing Group I Intron Ribozymes.Molecules (Basel, Switzerland) · 2017Review
- Article
- Effective suppression of dengue virus using a novel group-I intron that induces apoptotic cell death upon infection through conditional expression of the Bax C-terminal domain.Virology journal · 2014Article
- Bacterial group I introns: mobile RNA catalysts.Mobile DNA · 2014Article
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- Article
- Low selection pressure aids the evolution of cooperative ribozyme mutations in cells.The Journal of biological chemistry · 2013Article
- UU/UA dinucleotide frequency reduction in coding regions results in increased mRNA stability and protein expression.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- An in vivo selection method to optimize trans-splicing ribozymes.RNA (New York, N.Y.) · 2012Article
- Targeting of highly conserved Dengue virus sequences with anti-Dengue virus trans-splicing group I introns.BMC molecular biology · 2010Article
- Article
- Imaging Tetrahymena ribozyme splicing activity in single live mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The ability to form full-length intron RNA circles is a general property of nuclear group I introns.RNA (New York, N.Y.) · 2003Article
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo selection was used to improve the activity of the Tetrahymena pre-rRNA self-splicing intron in the context of heterologous exons. The intron was engineered into a kanamycin nucleotidyltransferase gene, with the pairing between intron bases and the 5' and 3' splice sites maintained. The initial construct failed to confer kanamycin resistance on Escherichia coli, although the pre-mRNA was active in splicing in vitro. Random mutation libraries were constructed to identify active intron variants in E. coli. All the active mutants sequenced contained mutations disrupting a base-paired region above the paired region P1 (referred to as the P1 extension region or P1ex) that involves the very 5' end of the intron. Subsequent site-directed mutagenesis confirmed that these P1ex mutations are responsible and sufficient to activate the intron splicing in E. coli. Thus, it appears that too strong of a secondary structure in the P1ex element can be inhibitory to splicing in vivo. In vitro splicing assays demonstrated that two P1ex mutant constructs splice six to eight times faster than the designed construct at 40 microM GTP concentration. The relative reaction rates of the mutant constructs compared to the original design are further increased at a lower GTP concentration. Possible mechanisms by which the disrupted P1ex structure could influence splicing rates are discussed. This study emphasizes the value of using libraries of random mutations to improve the activity of ribozymes in heterologous contexts in vivo.
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