ArticleRNA (New York, N.Y.)2002
Crosslinking of 4.5S RNA to the Escherichia coli ribosome in the presence or absence of the protein Ffh.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- A genomic-library based discovery of a novel, possibly synthetic, acid-tolerance mechanism in Clostridium acetobutylicum involving non-coding RNAs and ribosomal RNA processing.Metabolic engineering · 2010Article
- Transient tether between the SRP RNA and SRP receptor ensures efficient cargo delivery during cotranslational protein targeting.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Conformation of the signal recognition particle in ribosomal targeting complexes.RNA (New York, N.Y.) · 2009Article
- RNA crosslinking methods.Methods in enzymology · 2009Article
- Efficient interaction between two GTPases allows the chloroplast SRP pathway to bypass the requirement for an SRP RNA.Molecular biology of the cell · 2007Article
- The structure of Escherichia coli signal recognition particle revealed by scanning transmission electron microscopy.Molecular biology of the cell · 2006Article
- Conformation of 4.5S RNA in the signal recognition particle and on the 30S ribosomal subunit.RNA (New York, N.Y.) · 2005Article
- Interplay of signal recognition particle and trigger factor at L23 near the nascent chain exit site on the Escherichia coli ribosome.The Journal of cell biology · 2003Article
- The signal recognition particle binds to protein L23 at the peptide exit of the Escherichia coli ribosome.RNA (New York, N.Y.) · 2003Article
- Structure and functional properties of prokaryotic small noncoding RNAs.Folia microbiologica · 2003Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Radioactively labeled 4.5S RNA containing statistically distributed 4-thiouridine residues in place of normal uridine was prepared by T7 transcription. The ability of this modified 4.5S RNA to form a complex with the protein Ffh was demonstrated by a gel shift assay. The modified 4.5S RNA, with or without Ffh, was added to Escherichia coli ribosomes under various conditions, and crosslinking from the thiouridine residues was induced by irradiation at 350 nm. The crosslinked ribosomal components were analyzed by our standard procedures. Two clearly defined types of crosslinking were observed. The first was a crosslink to 23S rRNA, which was entirely dependent both on the presence of Ffh and a nascent protein chain in the 50S subunit. This crosslink was localized to nt approximately 2828-2837 of the 23S rRNA, from position 84 of the 4.5S molecule. The second type of crosslinking, to the 30S ribosomal subunit, was independent of the presence of Ffh, and was found both with vacant 70S ribosomes or isolated 30S subunits. Here the crosslink was localized to the 3'-terminal region of the 16S rRNA, from positions 29-50 of the 4.5S RNA. Cross-linking to ribosomal protein S1 was also observed. The known crystal structure of the protein Ffh/4.5S RNA fragment complex was extrapolated by computer modeling so as to include the whole 4.5S molecule, and this was docked onto the ribosome using the crosslinking data. The results are discussed in terms of multiple functions and binding sites of the 4.5S RNA.
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