Evidence map›Paper›PMID 12021244›Full record

ArticleThe Journal of clinical investigation2002

Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells.

Robert E Schwartz, Morayma Reyes, Lisa Koodie, Yuehua Jiang, Mark Blackstad, Troy Lund, Todd Lenvik, Sandra Johnson, Wei-Shou Hu, Catherine M Verfaillie

2 registry-linked trialsOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of clinical investigation, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 298 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
298citing papers in PubMed, 1 pooled it
38.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05165459 naunknown statusnot on this mapstarted 2021, after this paper: background citation

Open Label Single Arm Proof of Concept Trial to Evaluate the Efficacy and Safety of Cytori Celution System in Chronic Non-Healing Venous Leg Ulcers

TypeinterventionalSponsorSzeged UniversityRan2021 to 2022Enrolled10ConditionsVenous Leg Ulcer, Autologous Adipose Stromal Vascular Fraction, Adipose-Derived Mesenchymal Stem Cells, Mesenchymal Stem CellsArmsVenous leg ulcer treatment with adipous SVF
NCT05274295 naunknown statusnot on this mapstarted 2022, after this paper: background citation

Open Label Single Arm Proof of Concept Trial to Evaluate the Efficacy and Safety of Cytori Celution System in Diabetic Leg Ulcers

TypeinterventionalSponsorSzeged UniversityRan2022 to 2022Enrolled5ConditionsDiabetic Foot Ulcer, Leg Ulcer, Autologous Adipose Stromal Vascular Fraction, Mesenchymal Stem CellsArmsDiabetic leg ulcer treatment with adipous SVF
3 · Its place in the literature

Who cites it

298 citing papers in PubMed, 1 synthesis or guideline pooled it, 1,033 citations in OpenAlex.

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238 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 3 countries.

Robert E SchwartzStem Cell Institute, University of Minnesota, Minneapolis 55455, USA.
Morayma Reyes
Lisa Koodie
Yuehua Jiang
Mark Blackstad
Troy Lund
Todd Lenvik
Sandra Johnson
Wei-Shou Hu
Catherine M Verfaillie
University of Minnesota · USNHS Blood and Transplant · GB

Funding

NIAID NIH HHS F31AI/GM10291NIDDK NIH HHS R01-DK67932NIDDK NIH HHS U19-DK-61224
6 · The paper itself

Abstract

We have derived from normal human, mouse, and rat postnatal bone marrow primitive, multipotent adult progenitor cells (MAPCs) that can differentiate into most mesodermal cells and neuroectodermal cells in vitro and into all embryonic lineages in vivo. Here, we show that MAPCs can also differentiate into hepatocyte-like cells in vitro. Human, mouse, and rat MAPCs, cultured on Matrigel with FGF-4 and HGF, differentiated into epithelioid cells that expressed hepatocyte nuclear factor-3beta (HNF-3beta), GATA4, cytokeratin 19 (CK19), transthyretin, and alpha-fetoprotein by day 7, and expressed CK18, HNF-4, and HNF-1alpha on days 14-28. Virtually all human, as well as a majority of rodent cells stained positive for albumin and CK18 on day 21; 5% (rodent) to 25% (human) cells were binucleated by day 21. These cells also acquired functional characteristics of hepatocytes: they secreted urea and albumin, had phenobarbital-inducible cytochrome p450, could take up LDL, and stored glycogen. MAPCs, which can be expanded in vitro and maintained in an undifferentiated state for more than 100 population doublings, can thus differentiate into cells with morphological, phenotypic, and functional characteristics of hepatocytes. MAPCs may therefore be an ideal cell for in vivo therapies for liver disorders or for use in bioartificial liver devices.

Indexed as

Cell DifferentiationAdultAnimalsBiomarkersBone Marrow CellsCell LineageCells, CulturedHematopoietic Stem CellsHepatocytesHumansImmunohistochemistryMiceRatsBiomarkers

Identifiers

PMID12021244
PMCPMC150983
OpenAlexW2109191124

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.