ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2002
Nerve growth factor-induced differentiation changes the cellular organization of regulated Peptide release by PC12 cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- PKM2 from hyperactive neuron aggravate lung injury post-stroke by promoting FOXO3A/TXNIP mitochondria translocation in mice.Redox biology · 2026Article
- Useful Caged Compounds for Cell Physiology.Accounts of chemical research · 2020Article
- Mycalolide B dissociates dynactin and abolishes retrograde axonal transport of dense-core vesicles.Molecular biology of the cell · 2015Article
- Sorting of the neuroendocrine secretory protein Secretogranin II into the regulated secretory pathway: role of N- and C-terminal alpha-helical domains.The Journal of biological chemistry · 2008Article
- Novel approach to real-time flash photolysis and confocal [Ca2+] imaging.Pflugers Archiv : European journal of physiology · 2007Article
- Visualization of regulated exocytosis with a granule-membrane probe using total internal reflection microscopy.Molecular biology of the cell · 2004Article
- Unexpected mobility variation among individual secretory vesicles produces an apparent refractory neuropeptide pool.Biophysical journal · 2003Article
- Physical mobilization of secretory vesicles facilitates neuropeptide release by nerve growth factor-differentiated PC12 cells.The Journal of physiology · 2002Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
PC12 cells, like endocrine chromaffin cells, undergo neuronal-like differentiation in response to nerve growth factor (NGF). Here we report that this phenotype conversion produces major changes in release of a green fluorescent protein-tagged neuropeptide-hormone. First, the spatial distribution of the releasable pool is altered; peptide release from untreated cells is supported predominantly by membrane-proximal vesicles, whereas a diffuse pool at the ends of processes is used by NGF-treated cells. Second, the time course of release evoked by photolysis of caged Ca(2+) is faster after differentiation. High-resolution measurements suggest that a slow step before membrane fusion dominates the kinetics of release in untreated cells. Finally, the effect of actin microfilament depolymerization on total release is altered by NGF treatment. This implies that the mechanism that limits the size of the releasable pool is altered by phenotype conversion. Therefore, the cellular organization of peptide release is plastic and changes in response to NGF. This flexibility may be used to generate cell-specific release properties.
Indexed as
Identifiers
12019308PMC6757627W2107299309What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.