ArticleAntimicrobial agents and chemotherapy2002
Internal thiols and reactive oxygen species in candidacidal activity exerted by an N-terminal peptide of human lactoferrin.
Article in Antimicrobial agents and chemotherapy, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 60 citations in OpenAlex.
- Antifungal Activity of Cedrol fromPlants (Basel, Switzerland) · 2024Article
- Synergistic Activity of the Human Lactoferricin-Derived Peptide hLF1-11 in Combination with Caspofungin againstMicrobiology spectrum · 2022Article
- A Novel Dual-Targeted α-Helical Peptide With Potent Antifungal Activity Against Fluconazole-ResistantFrontiers in microbiology · 2020Article
- Insight into the Antifungal Mechanism of Action of Human RNase N-terminus Derived Peptides.International journal of molecular sciences · 2019Article
- Article
- Antimicrobial Activity of Lactoferrin-Related Peptides and Applications in Human and Veterinary Medicine.Molecules (Basel, Switzerland) · 2016Review
- Antifungal Activity and Action Mechanism of Histatin 5-Halocidin Hybrid Peptides against Candida ssp.PloS one · 2016Article
- Inhibition of Candida albicans Biofilm Formation by the Synthetic Lactoferricin Derived Peptide hLF1-11.PloS one · 2016Article
- Synthesis of a New Peptide-Coumarin Conjugate: A Potential Agent against Cryptococcosis.ACS medicinal chemistry letters · 2015Article
- Evaluation of Oxidative DNA Damage Using an Alkaline Single Cell Gel Electrophoresis (SCGE) Comet Assay, and the Protective Effects of N-Acetylcysteine Amide on Zearalenone-induced Cytotoxicity in Chang Liver Cells.Toxicological research · 2013Article
- Involvement of the mitogen activated protein kinase Hog1p in the response of Candida albicans to iron availability.BMC microbiology · 2013Article
- Pro-oxidant and antioxidant effects of N-acetylcysteine regulate doxorubicin-induced NF-kappa B activity in leukemic cells.Molecular bioSystems · 2012Article
- N-acetylcysteine amide decreases oxidative stress but not cell death induced by doxorubicin in H9c2 cardiomyocytes.BMC pharmacology · 2009Article
- The role of released ATP in killing Candida albicans and other extracellular microbial pathogens by cationic peptides.Purinergic signalling · 2007Article
- Peptide antimicrobial agents.Clinical microbiology reviews · 2006Review
- MNN5 encodes an iron-regulated alpha-1,2-mannosyltransferase important for protein glycosylation, cell wall integrity, morphogenesis, and virulence in Candida albicans.Eukaryotic cell · 2006Article
- Reactive oxygen species play no role in the candidacidal activity of the salivary antimicrobial peptide histatin 5.The Biochemical journal · 2004Article
- Interactions of histatin 5 and histatin 5-derived peptides with liposome membranes: surface effects, translocation and permeabilization.The Biochemical journal · 2004Article
- One of two human lactoferrin variants exhibits increased antibacterial and transcriptional activation activities and is associated with localized juvenile periodontitis.Infection and immunity · 2003Article
- Synergistic activity of the N-terminal peptide of human lactoferrin and fluconazole against Candida species.Antimicrobial agents and chemotherapy · 2003Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously showed that the energized mitochondrion and extracellular ATP are essential for the candidacidal activity of the N-terminal peptide of human lactoferrin, subsequently referred to as hLF(1-11). The present study focuses on the involvement of internal thiols and reactive oxygen species (ROS) in the candidacidal activity exerted by hLF(1-11). Our results reveal that hLF(1-11) reduced the internal thiol level of Candida albicans by 20%. In agreement, N-acetyl-L-cysteine (NAC), which is a precursor of glutathione and an ROS scavenger, inhibited the candidacidal activity of hLF(1-11). In addition, azodicarboxylic acid bis(N,N-dimethylamide) (diamide), which oxidizes internal thiols, was candidacidal. Furthermore, hLF(1-11) increased the level of ROS production by C. albicans in a dose-dependent manner, and a correlation between ROS production and candidacidal activity was found. 6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox), which is an ROS scavenger, partially inhibited the hLF(1-11)-induced, but not the diamide-triggered, candidacidal activity. It is of interest that hLF(1-11) and diamide acted synergistically in killing C. albicans and in ROS production. In agreement, oxidized ATP, an irreversible inhibitor of extracellular ATP receptors, partially blocked the hLF(1-11)-induced, but not the diamide-triggered, candidacidal activity. Finally, the hLF(1-11)-induced activation of mitochondria was inhibited by NAC, indicating that internal thiols and ROS affect mitochondrial activity. Therefore, the candidacidal activity of hLF(1-11) involves both generation of ROS and reduction of internal thiols.
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