ArticlePlant physiology2002
The 5'-untranslated region of the ntp303 gene strongly enhances translation during pollen tube growth, but not during pollen maturation.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 65 citations in OpenAlex.
- Evolutionary conservation of nested MIR159 structural microRNA genes and their promoter characterization inFrontiers in plant science · 2022Article
- A novel adenylate isopentenyltransferase 5 regulates shoot branching via the ATTTA motif in Camellia sinensis.BMC plant biology · 2021Article
- A Maize Male Gametophyte-Specific Gene Encodes ZmLARP6c1, a Potential RNA-Binding Protein Required for Competitive Pollen Tube Growth.Frontiers in plant science · 2021Article
- Article
- bHLH142 regulates various metabolic pathway-related genes to affect pollen development and anther dehiscence in rice.Scientific reports · 2017Article
- 5'-non-transcribed flanking region and 5'-untranslated region play distinctive roles in sulfur deficiency induced expression ofPlant biotechnology (Tokyo, Japan) · 2017Article
- Genome-wide conserved non-coding microsatellite (CNMS) marker-based integrative genetical genomics for quantitative dissection of seed weight in chickpea.Journal of experimental botany · 2015Article
- RNA-Seq SSRs of Moth Orchid and Screening for Molecular Markers across Genus Phalaenopsis (Orchidaceae).PloS one · 2015Article
- Large scale in-silico identification and characterization of simple sequence repeats (SSRs) from de novo assembled transcriptome of Catharanthus roseus (L.) G. Don.Plant cell reports · 2014Article
- Translational Regulation of Cytoplasmic mRNAs.The arabidopsis book · 2013Article
- Truncated cotton subtilase promoter directs guard cell-specific expression of foreign genes in tobacco and Arabidopsis.PloS one · 2013Article
- The 5' untranslated region of the soybean cytosolic glutamine synthetase β(1) gene contains prokaryotic translation initiation signals and acts as a translational enhancer in plants.Molecular genetics and genomics : MGG · 2012Article
- Known and novel post-transcriptional regulatory sequences are conserved across plant families.RNA (New York, N.Y.) · 2012Article
- Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar cell expansion and underlying heterochronic shift during spermatogenesis.BMC plant biology · 2012Article
- Evaluation of seed storage-protein gene 5' untranslated regions in enhancing gene expression in transgenic rice seed.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Article
- Conservation of noncoding microsatellites in plants: implication for gene regulation.BMC genomics · 2006Article
- Isolation and characterisation of three moss-derived beta-tubulin promoters suitable for recombinant expression.Current genetics · 2005Article
- The putative Arabidopsis homolog of yeast vps52p is required for pollen tube elongation, localizes to Golgi, and might be involved in vesicle trafficking.Plant physiology · 2004Article
- Silencing of the pollen-specific gene NTP303 and its family members in tobacco affects in vivo pollen tube growth and results in male sterile plants.Plant molecular biology · 2004Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcripts of the ntp303 gene accumulate abundantly throughout pollen development, whereas the protein only accumulates to detectable levels after pollen germination. In an attempt to explain the divergence in the accumulation profiles of the mRNA and the protein, we investigated the role of the untranslated regions (UTRs) in enhancing ntp303 translation during the transition from developing to germinating pollen. Luciferase reporter gene fusion constructs containing the ntp303 5'-UTR gave rise to luciferase activity that was up to 60-fold higher during pollen tube growth than that of constructs containing different 5'-UTRs. No apparent differences in the luciferase activity of these constructs were observed during pollen development. The ntp303 5'-UTR-mediated increase in luciferase activity was not significantly influenced by coding region or 3'-UTR sequences. Furthermore, enhanced luciferase activity directed by the ntp303 5'-UTR occurred predominantly at the post-transcriptional level. A series of 5'-UTR deletion constructs was created to identify putative regulatory sequences required for the high level of translation during pollen tube growth. Two predicted stem loop structures (H-I and H-II) caused a complete inhibition of the enhanced translation after their total or partial deletion. A (GAA)(8) repeat within the H-I stem loop structure was demonstrated to be important for the modulation of translation efficiency. The H-II stem loop structure was found to be essential for the determination of mRNA stability.
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