ArticlePlant physiology2002
Glucosylation activity and complex formation of two classes of reversibly glycosylated polypeptides.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
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- Full-length transcriptome analysis of shade-induced promotion of tuber production in Pinellia ternata.BMC plant biology · 2019Article
- Plasmodesmata Conductivity Regulation: A Mechanistic Model.Plants (Basel, Switzerland) · 2019Review
- Molecular characteristics of plant UDP-arabinopyranose mutases.Glycobiology · 2019Review
- X-ray diffraction analysis and in vitro characterization of the UAM2 protein from Oryza sativa.Acta crystallographica. Section F, Structural biology communications · 2017Article
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- Systems Genetics Identifies a Novel Regulatory Domain of Amylose Synthesis.Plant physiology · 2017Article
- The Cell Wall Arabinose-Deficient Arabidopsis thaliana Mutant murus5 Encodes a Defective Allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2.Plant physiology · 2016Article
- Formation of starch in plant cells.Cellular and molecular life sciences : CMLS · 2016Review
- Getting ready for host invasion: elevated expression and action of xyloglucan endotransglucosylases/hydrolases in developing haustoria of the holoparasitic angiosperm Cuscuta.Journal of experimental botany · 2016Article
- Cytological and proteomic analyses of horsetail (Equisetum arvense L.) spore germination.Frontiers in plant science · 2015Article
- Callose homeostasis at plasmodesmata: molecular regulators and developmental relevance.Frontiers in plant science · 2014Article
- Transcriptome analysis of shade-induced inhibition on leaf size in relay intercropped soybean.PloS one · 2014Article
- Starch synthase 4 is essential for coordination of starch granule formation with chloroplast division during Arabidopsis leaf expansion.The New phytologist · 2013Article
- Oligomerization of the reversibly glycosylated polypeptide: its role during rice plant development and in the regulation of self-glycosylation.Protoplasma · 2013Article
- Control of starch granule numbers in Arabidopsis chloroplasts.Plant physiology · 2012Article
- Reduced levels of class 1 reversibly glycosylated polypeptide increase intercellular transport via plasmodesmata.Plant signaling & behavior · 2012Article
- The interconversion of UDP-arabinopyranose and UDP-arabinofuranose is indispensable for plant development in Arabidopsis.The Plant cell · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reversibly glycosylated polypeptides (RGPs) have been implicated in polysaccharide biosynthesis. In plants, these proteins may function, for example, in cell wall synthesis and/or in synthesis of starch. We have isolated wheat (Triticum aestivum) and rice (Oryza sativa) Rgp cDNA clones to study the function of RGPs. Sequence comparisons showed the existence of two classes of RGP proteins, designated RGP1 and RGP2. Glucosylation activity of RGP1 and RGP2 from wheat and rice was studied. After separate expression of Rgp1 and Rgp2 in Escherichia coli or yeast (Saccharomyces cerevisiae), only RGP1 showed self-glucosylation. In Superose 12 fractions from wheat endosperm extract, a polypeptide with a molecular mass of about 40 kD is glucosylated by UDP-glucose. Transgenic tobacco (Nicotiana tabacum) plants, overexpressing either wheat Rgp1 or Rgp2, were generated. Subsequent glucosylation assays revealed that in RGP1-containing tobacco extracts as well as in RGP2-containing tobacco extracts UDP-glucose is incorporated, indicating that an RGP2-containing complex is active. Gel filtration experiments with wheat endosperm extracts and extracts from transgenic tobacco plants, overexpressing either wheat Rgp1 or Rgp2, showed the presence of RGP1 and RGP2 in high-molecular mass complexes. Yeast two-hybrid studies indicated that RGP1 and RGP2 form homo- and heterodimers. Screening of a cDNA library using the yeast two-hybrid system and purification of the complex by an antibody affinity column did not reveal the presence of other proteins in the RGP complexes. Taken together, these results suggest the presence of active RGP1 and RGP2 homo- and heteromultimers in wheat endosperm.
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