ArticlePlant physiology2002
Expression, activation, and biochemical properties of a novel Arabidopsis protein kinase.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Unusual metal ion cofactor requirement of Entamoeba histolytica choline and ethanolamine kinase isoforms.Parasitology research · 2023Article
- Cold Tolerance ofCurrent issues in molecular biology · 2022Article
- Plant Calcium Signaling in Response to Potassium Deficiency.International journal of molecular sciences · 2018Review
- CIPK9 is involved in seed oil regulation inBiotechnology for biofuels · 2018Article
- Phosphorylation of the Brome Mosaic Virus Capsid Regulates the Timing of Viral Infection.Journal of virology · 2016Article
- Overexpression ofPlant molecular biology reporter · 2014Article
- Arabidopsis CBL-interacting protein kinase (CIPK6) is involved in plant response to salt/osmotic stress and ABA.Molecular biology reports · 2013Article
- Suppressor of K+ transport growth defect 1 (SKD1) interacts with RING-type ubiquitin ligase and sucrose non-fermenting 1-related protein kinase (SnRK1) in the halophyte ice plant.Journal of experimental botany · 2013Article
- The Brassica napus calcineurin B-Like 1/CBL-interacting protein kinase 6 (CBL1/CIPK6) component is involved in the plant response to abiotic stress and ABA signalling.Journal of experimental botany · 2012Article
- Cloning and characterization of a maize SnRK2 protein kinase gene confers enhanced salt tolerance in transgenic Arabidopsis.Plant cell reports · 2011Article
- Article
- Arabidopsis protein kinase PKS5 inhibits the plasma membrane H+ -ATPase by preventing interaction with 14-3-3 protein.The Plant cell · 2007Article
- A rice dehydration-inducible SNF1-related protein kinase 2 phosphorylates an abscisic acid responsive element-binding factor and associates with ABA signaling.Plant molecular biology · 2007Article
- Role of an Arabidopsis AP2/EREBP-type transcriptional repressor in abscisic acid and drought stress responses.The Plant cell · 2005Article
- Identification and gene expression analysis of a large family of transmembrane kinases related to the Gal/GalNAc lectin in Entamoeba histolytica.Eukaryotic cell · 2005Article
- Biochemical characterization of the tobacco 42-kD protein kinase activated by osmotic stress.Plant physiology · 2004Article
- Integration and channeling of calcium signaling through the CBL calcium sensor/CIPK protein kinase network.Planta · 2004Review
- The SOS3 family of calcium sensors and SOS2 family of protein kinases in Arabidopsis.Plant physiology · 2004Review
- Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks.Plant physiology · 2004Article
- Isolation and characterization of a novel rice Ca2+-regulated protein kinase gene involved in responses to diverse signals including cold, light, cytokinins, sugars and salts.Plant molecular biology · 2003Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
An Arabidopsis SOS2 (salt overly sensitive 2)-like protein kinase gene, PKS6, was expressed in leaves, stems, and siliques, but not detectable in roots of adult plants; its expression in young seedlings was up-regulated by abscisic acid. To determine the biochemical properties of the PKS6 protein, we expressed the PKS6 coding sequence as a glutathione S-transferase fusion protein in Escherichia coli. The bacterially expressed glutathione S-transferase-PKS6 fusion protein was inactive in substrate phosphorylation. We have constructed constitutively active forms of PKS6 by either a deletion of its putative auto-inhibitory FISL motif (i.e. PKS6deltaF) or a substitution of threonine-178 with aspartic acid within the putative activation loop. We found that PKS6deltaF exhibited a strong preference for Mn2+ over Mg2+ as a divalent cation cofactor for kinase activity. PKS6DeltaF displayed substrate specificity against three different peptide substrates and had an optimal pH of approximately 7.5 and temperature optimum of 30 degrees C. The apparent Km values for ATP and the preferred peptide substrate p3 of PKS6deltaF were determined to be 1.7 and 28.5 microM, respectively. These results provide significant insights into the regulation and biochemical properties of the protein kinase PKS6. In addition, the constitutively active, gain-of-function kinase mutants will be invaluable for future determination of the in planta function of PKS6.
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