ArticlePlant physiology2002
LeCPK1, a calcium-dependent protein kinase from tomato. Plasma membrane targeting and biochemical characterization.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 104 citations in OpenAlex.
- Article
- Calcium-Dependent Protein Kinase GhCDPK16 Exerts a Positive Regulatory Role in Enhancing Drought Tolerance in Cotton.International journal of molecular sciences · 2024Article
- Identification and expression analysis of calcium-dependent protein kinase family in oat (Frontiers in plant science · 2024Article
- Functional characterization and structural basis of a reversible glycosyltransferase involves in plant chemical defence.Plant biotechnology journal · 2023Article
- Identification of CDK gene family and functional analysis of CqCDK15 under drought and salt stress in quinoa.BMC genomics · 2023Article
- The putative myristoylome of Physcomitrium patens reveals conserved features of myristoylation in basal land plants.Plant cell reports · 2023Article
- Molecular Analysis of MgO Nanoparticle-Induced Immunity against Fusarium Wilt in Tomato.International journal of molecular sciences · 2023Article
- The role of CDPKs in plant development, nutrient and stress signaling.Frontiers in genetics · 2022Review
- Proteins with calmodulin-like domains: structures and functional roles.Cellular and molecular life sciences : CMLS · 2019Review
- Targeted Profiling ofThe Plant cell · 2018Article
- Activation of a calcium-dependent protein kinase involved in the Azospirillum growth promotion in rice.World journal of microbiology & biotechnology · 2017Article
- Analysis of EF-Hand Proteins in Soybean Genome Suggests Their Potential Roles in Environmental and Nutritional Stress Signaling.Frontiers in plant science · 2017Article
- Genome-wide analysis of calcium-dependent protein kinases and their expression patterns in response to herbivore and wounding stresses in soybean.Functional & integrative genomics · 2016Article
- Cell Death Control by Matrix Metalloproteinases.Plant physiology · 2016Article
- Genome-wide identification of calcium-dependent protein kinases in soybean and analyses of their transcriptional responses to insect herbivory and drought stress.Scientific reports · 2016Article
- Holophytochrome-Interacting Proteins in Physcomitrella: Putative Actors in Phytochrome Cytoplasmic Signaling.Frontiers in plant science · 2016Article
- A Role for Barley Calcium-Dependent Protein Kinase CPK2a in the Response to Drought.Frontiers in plant science · 2016Article
- Genome-Wide Identification and Expression Analysis of Calcium-dependent Protein Kinase in Tomato.Frontiers in plant science · 2016Article
- Ancient signals: comparative genomics of green plant CDPKs.Trends in plant science · 2014Review
- Microarray analysis of tomato plants exposed to the nonviruliferous or viruliferous whitefly vector harboring Pepper golden mosaic virus.Journal of insect science (Online) · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cDNA of LeCPK1, a calcium-dependent protein kinase, was cloned from tomato (Lycopersicon esculentum Mill.). LeCPK1 was expressed in Escherichia coli and purified from bacterial extracts. The recombinant protein was shown to be a functional protein kinase using a synthetic peptide as the substrate (syntide-2, Km = 85 microM). Autophosphorylation of LeCPK1 was observed on threonine and serine residues, one of which was identified as serine-439. Kinase activity was shown to be Ca2+ dependent and required the C-terminal, calmodulin-like domain of LeCPK1. Two classes of high- and low-affinity Ca2+-binding sites were observed, exhibiting dissociation constants of 0.6 and 55 microM, respectively. LeCPK1 was found to phosphorylate the regulatory C-terminal domain of the plasma membrane H+-ATPase in vitro. A potential role in the regulation of proton pump activity is corroborated by the apparent colocalization of the plasma membrane H+-ATPase and LeCPK1 in vivo. Upon transient expression in suspension-cultured cells, a C-terminal fusion of LeCPK1 with the green fluorescent protein was targeted to the plasma membrane. Myristoylation of the LeCPK1 N terminus was found to be required for plasma membrane targeting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.