ArticlePlant physiology2002
Phospholipase A2 activity triggers the wound-activated chemical defense in the diatom Thalassiosira rotula.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 204 citations in OpenAlex.
- Rapid adaptive evolution of oxylipin-based chemical defense against algicidal bacteria in a bloom-forming diatom.The ISME journal · 2026Article
- Reactive oxygen species trigger downward vertical migration in diatom microphytobenthic biofilms as a strategy to cope with oxidative stress.The ISME journal · 2026Article
- Mapping the transcriptional landscape of algal resistance to viral infection reveals a core expression program.The New phytologist · 2025Article
- Lipidomic Screening of Marine Diatoms Reveals Release of Dissolved Oxylipins Associated with Silicon Limitation and Growth Phase.Marine drugs · 2025Article
- Metabolic changes and biochemical degradation during dark anoxic incubation of Nannochloropsis: implications for low-energy microalgal cell rupture.Bioprocess and biosystems engineering · 2025Article
- Stress-Induced Production of Bioactive Oxylipins in Marine Microalgae.Marine drugs · 2024Article
- Untargeted metabolomics to expand the chemical space of the marine diatomFrontiers in microbiology · 2023Article
- Resolving the Microalgal Gene Landscape at the Strain Level: a Novel Hybrid Transcriptome ofApplied and environmental microbiology · 2022Article
- Density-Dependent Prophylaxis in Freshwater Snails Driven by Oxylipin Chemical Cues.Frontiers in immunology · 2022Article
- Inclusion of photoautotrophic cultivated diatom biomass in salmon feed can deter lice.PloS one · 2021Article
- Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption.Scientific reports · 2020Article
- trans, trans-2,4-Decadienal, a lipid peroxidation product, induces inflammatory responses via Hsp90- or 14-3-3ζ-dependent mechanisms.The Journal of nutritional biochemistry · 2020Article
- Combined Effects of Diatom-Derived Oxylipins on the Sea UrchinInternational journal of molecular sciences · 2020Article
- Lipoxygenase Pathways in Diatoms: Occurrence and Correlation with Grazer Toxicity in Four Benthic Species.Marine drugs · 2020Article
- Density-dependent oxylipin production in natural diatom communities: possible implications for plankton dynamics.The ISME journal · 2020Article
- Distinctive Growth and Transcriptional Changes of the DiatomFrontiers in microbiology · 2020Article
- Cell Death in Evolutionary Transitions in Individuality.The Yale journal of biology and medicine · 2019Review
- Microalgal Enzymes with Biotechnological Applications.Marine drugs · 2019Review
- Molecular and Morphological Toxicity of Diatom-Derived Hydroxyacid Mixtures to Sea UrchinMarine drugs · 2019Article
- Associated Bacteria Affect Sexual Reproduction by Altering Gene Expression and Metabolic Processes in a Biofilm Inhabiting Diatom.Frontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The activation of oxylipin-based chemical defense in the diatom Thalassiosira rotula is initiated by phospholipases that act immediately after cell damage. This lipase activity is responsible for the preferential release of free mono- and polyunsaturated fatty acids. Among these, eicosatetraenoic- and eicosapentaenoic acid are further converted by lipoxygenases to reactive defensive metabolites such as the antiproliferative alpha,beta,gamma,delta-unsaturated aldehydes 2,4-decadienal and 2,4,7-decatrienal. We show that mainly saturated free fatty acids are present in the intact diatom T. rotula, whereas the amount of free polyunsaturated eicosanoids is drastically increased in the first minutes after wounding. Using fluorescent probes, the main enzyme activity responsible for initiation of the aldehyde-generating lipase/lipoxygenase/hydroperoxide lyase cascade was characterized as a phospholipase A2. All enzymes involved in this specific defensive reaction are active in seawater over several minutes. Thus, the mechanism allows the unicellular algae to overcome restrictions arising out of potential dilution of defensive metabolites. Only upon predation are high local concentrations of aldehydes formed in the vicinity of the herbivores, whereas in times of low stress, cellular resources can be invested in the formation of eicosanoid-rich phospholipids. In contrast to higher plants, which use lipases acting on galactolipids to release C18 fatty acids for production of leaf-volatile aldehydes, diatoms rely on phospholipids and the transformation of C20 fatty acids to form 2,4-decadienal and 2,4,7-decatrienal as an activated defense.
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