Evidence map›Paper›PMID 12011021›Full record

ArticleInfection and immunity2002

Entamoeba histolytica lectins contain unique 6-Cys or 8-Cys chitin-binding domains.

Katrina Van Dellen, Sudip K Ghosh, Phillips W Robbins, Brendan Loftus, John Samuelson

Open access · bronzeAbstract read
In one paragraph

Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 45 citations in OpenAlex.

  1. Article
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  9. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Katrina Van DellenDepartment of Immunology and Infectious Diseases, Harvard School of Public Health, Boston University School of Dental Medicine, Boston, Massachusetts 02115, USA.
Sudip K Ghosh
Phillips W Robbins
Brendan Loftus
John Samuelson
Harvard University · USBoston University · US

Funding

ROBBINS Glycosylation and Glycosidases-Cell and Molecular BiologyR01GM031318 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI SAMUELSON, JOHN C. · 1985 to 2011
$4.1M
ENTAMOEBA HISTOLYTICA GENOME PROJECTR01AI046516 · NIAID · INSTITUTE FOR GENOMIC RESEARCH · PI LOFTUS, BRENDAN · 2000 to 2002
$1.9M
ALCOHOL DEHYDROGENASES OF ENTAMOEBAER01AI033492 · NIAID · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI SAMUELSON, JOHN C. · 1995 to 1998
–
PROTEIN GLYCOSYLATION - CELLULAR AND MOLECULAR BIOLOGYR37GM031318 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI ROBBINS, PHILLIPS W · 1988 to 1997
–
NIAID NIH HHS AI33492NIAID NIH HHS AI46516NIAID NIH HHS R01 AI046516NIGMS NIH HHS GM31318NIGMS NIH HHS R01 GM031318
6 · The paper itself

Abstract

The Jacob lectin, the most abundant glycoprotein in the cyst wall of Entamoeba invadens, contains five unique 6-Cys chitin-binding domains (CBDs). We identified Entamoeba histolytica and Entamoeba dispar genes encoding Jacob homologues, each of which contains two predicted 6-Cys CBDs. A unique 8-Cys CBD was found at the N termini of the E. histolytica chitinase and three other predicted lectins, called Jessie 1 to Jessie 3. The CBDs of four E. histolytica lectins (Jacob, chitinase, and Jessies 2 and 3) were expressed in secretory vesicles of transfected amebae and shown to bind to particulate chitin.

Indexed as

Amino Acid SequenceAnimalsBase SequenceBinding SitesChitinChitinasesCysteineDNA, ProtozoanEntamoeba histolyticaGlycoproteinsLectinsMolecular Sequence DataProtozoan ProteinsChitinChitinasesCysteineDNA, ProtozoanGlycoproteinsJacob protein, Entamoeba histolyticaLectinsProtozoan Proteins

Identifiers

PMID12011021
PMCPMC127964
OpenAlexW2106787853

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.