ArticleInfection and immunity2002
Construction and phenotypic characterization of an auxotrophic mutant of Mycobacterium tuberculosis defective in L-arginine biosynthesis.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 88 citations in OpenAlex.
- Metabolic reprogramming of macrophages during mycobacterial infection: a review of immunometabolic crosstalk and pathogen manipulation.Frontiers in cellular and infection microbiology · 2026Review
- Mycobacterium tuberculosis curli pili facilitates pathogenicity by modulating central carbon metabolism.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- New insight into arginine and tryptophan metabolism in macrophage activation during tuberculosis.Frontiers in immunology · 2024Review
- Arginine Metabolism Powers Salmonella Resistance to Oxidative Stress.Infection and immunity · 2023Article
- Identification of small molecules targeting homoserine acetyl transferase from Mycobacterium tuberculosis and Staphylococcus aureus.Scientific reports · 2022Article
- Metabolite Dysregulation by Pranlukast inMolecules (Basel, Switzerland) · 2022Article
- Morphological and transcriptomic analysis of attenuated and virulent strains of Phytophthora infestans.International microbiology : the official journal of the Spanish Society for Microbiology · 2022Article
- Understanding the contribution of metabolism toFrontiers in cellular and infection microbiology · 2022Review
- Phenotypic characterization of auxotrophic mutant of nontyphoidal Salmonella and determination of its cytotoxicity, tumor inhibiting cytokine gene expression in cell line models.Archives of microbiology · 2021Article
- Multi-omic regulatory networks capture downstream effects of kinase inhibition in Mycobacterium tuberculosis.NPJ systems biology and applications · 2021Article
- Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways.mSystems · 2019Article
- Development of a whole-cell high-throughput phenotypic screen to identify inhibitors of mycobacterial amino acid biosynthesis.FASEB bioAdvances · 2019Article
- Arginine-deprivation-induced oxidative damage sterilizesProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Death ofProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- An allosteric inhibitor ofEMBO molecular medicine · 2018Article
- Review
- The Mycobacterium tuberculosis H37Ra gene MRA_1916 causes growth defects upon down-regulation.Scientific reports · 2015Article
- Essential roles of methionine and S-adenosylmethionine in the autarkic lifestyle of Mycobacterium tuberculosis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Mycobacterium tuberculosis Is a Natural Ornithine Aminotransferase (rocD) Mutant and Depends on Rv2323c for Growth on Arginine.PloS one · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A mutant of Mycobacterium tuberculosis defective in the metabolism of L-arginine was constructed by allelic exchange mutagenesis. The argF mutant strain required exogenous L-arginine for growth in vitro, and in the presence of 0.96 mM L-arginine, it achieved a growth rate and cell density in stationary phase comparable to those of the wild type. The mutant strain was also able to grow in the presence of high concentrations of argininosuccinate, but its auxotrophic phenotype could not be rescued by L-citrulline, suggesting that the DeltaargF::hyg mutation exerted a polar effect on the downstream argG gene but not on argH. The mutant strain displayed reduced virulence in immunodeficient SCID mice and was highly attenuated in immunocompetent DBA/2 mice, suggesting that L-arginine availability is restricted in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.