ArticleInfection and immunity2002
Modulation of Borrelia burgdorferi stringent response and gene expression during extracellular growth with tick cells.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Hitchhiker's Guide toJournal of bacteriology · 2024Review
- DksA-dependent regulation of RpoS contributes to Borrelia burgdorferi tick-borne transmission and mammalian infectivity.PLoS pathogens · 2021Article
- Ecological and evolutionary perspectives on tick-borne pathogen co-infections.Current research in parasitology & vector-borne diseases · 2021Review
- DksA plays an essential role in regulating the virulence of Borrelia burgdorferi.Molecular microbiology · 2020Article
- The intergenic small non-coding RNA ittA is required for optimal infectivity and tissue tropism in Borrelia burgdorferi.PLoS pathogens · 2020Article
- Sleeper cells: the stringent response and persistence in the Borreliella (Borrelia) burgdorferi enzootic cycle.Environmental microbiology · 2017Review
- Fluorescent membrane markers elucidate the association of Borrelia burgdorferi with tick cell lines.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2016Article
- Long-term survival of Borrelia burgdorferi lacking the hibernation promotion factor homolog in the unfed tick vector.Infection and immunity · 2015Article
- Characterization of the RelBbu Regulon in Borrelia burgdorferi Reveals Modulation of Glycerol Metabolism by (p)ppGpp.PloS one · 2015Article
- Coinfection of tick cell lines has variable effects on replication of intracellular bacterial and viral pathogens.Ticks and tick-borne diseases · 2014Article
- Patterns and regulation of ribosomal RNA transcription in Borrelia burgdorferi.BMC microbiology · 2011Article
- ppGpp conjures bacterial virulence.Microbiology and molecular biology reviews : MMBR · 2010Review
- Characterization of Borrelia burgdorferi aggregates.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2009Article
- Transposon insertion reveals pRM, a plasmid of Rickettsia monacensis.Applied and environmental microbiology · 2007Article
- Evolving models of Lyme disease spirochete gene regulation.Wiener klinische Wochenschrift · 2006Review
- Borrelia burgdorferi BmpA, BmpB, and BmpD proteins are expressed in human infection and contribute to P39 immunoblot reactivity in patients with Lyme disease.Clinical and diagnostic laboratory immunology · 2005Article
- Borrelia burgdorferi rel is responsible for generation of guanosine-3'-diphosphate-5'-triphosphate and growth control.Infection and immunity · 2005Article
- Localization of BmpA on the exposed outer membrane of Borrelia burgdorferi by monospecific anti-recombinant BmpA rabbit antibodies.Infection and immunity · 2004Article
- Characterization of the stringent response and rel(Bbu) expression in Borrelia burgdorferi.Journal of bacteriology · 2003Article
- How relevant are in vitro culture models for study of tick-pathogen interactions?Pathogens and global healthReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Borrelia burgdorferi N40 multiplied extracellularly when it was cocultured with tick cells in L15BS medium, a medium which by itself did not support B. burgdorferi N40 growth. Growth of B. burgdorferi N40 in the presence of tick cells was associated with decreased production of (p)ppGpp, the stringent response global regulator, a fourfold decrease in relA/spoT mRNA, an eightfold net decrease in bmpD mRNA, and a fourfold increase in rpsL-bmpD mRNA compared to growth of B. burgdorferi in BSK-H medium. As a result, the polycistronic rpsL-bmpD mRNA level increased from 3 to 100% of the total bmpD message. These observations demonstrate that there are reciprocal interactions between B. burgdorferi and tick cells in vitro and indicate that the starvation-associated stringent response mediated by (p)ppGpp present in B. burgdorferi growing in BSK-H medium is ameliorated in B. burgdorferi growing in coculture with tick cell lines. These results suggest that this system can provide a useful model for identifying genes controlling interactions of B. burgdorferi with tick cells in vitro when it is coupled with genetic methods to isolate and complement B. burgdorferi mutants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.