ArticleInfection and immunity2002
Proteomic analysis of differentially expressed Chlamydia pneumoniae genes during persistent infection of HEp-2 cells.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 86 citations in OpenAlex.
- Persistence of obligate intracellular pathogens: alternative strategies to overcome host-specific stresses.Frontiers in cellular and infection microbiology · 2023Review
- Evidence for vesicle-mediated antigen export by the human pathogenLife science alliance · 2019Article
- Review
- Tryptophan Codon-Dependent Transcription in Chlamydia pneumoniae during Gamma Interferon-Mediated Tryptophan Limitation.Infection and immunity · 2016Article
- Membrane vesicle production by Chlamydia trachomatis as an adaptive response.Frontiers in cellular and infection microbiology · 2014Review
- Persistent C. pneumoniae infection in atherosclerotic lesions: rethinking the clinical trials.Frontiers in cellular and infection microbiology · 2014Review
- Laboratory diagnosis of persistent human chlamydial infection.Frontiers in cellular and infection microbiology · 2013Review
- Altered developmental expression of polymorphic membrane proteins in penicillin-stressed Chlamydia trachomatis.Cellular microbiology · 2011Article
- Chlamydia pneumoniae-induced memory CD4+ T-cell activation in human peripheral blood correlates with distinct antibody response patterns.Clinical and vaccine immunology : CVI · 2010Article
- Kinematics of intracellular chlamydiae provide evidence for contact-dependent development.Journal of bacteriology · 2009Article
- Potential problems inherent in cell-based stable NF-kappaB-GFP reporter systems.Molecular and cellular biochemistry · 2008Article
- Proteomic analysis and identification of Streptococcus pyogenes surface-associated proteins.Journal of bacteriology · 2007Article
- Tissue MicroArray (TMA) analysis of normal and persistent Chlamydophila pneumoniae infection.BMC infectious diseases · 2006Article
- Transcriptional response patterns of Chlamydophila psittaci in different in vitro models of persistent infection.Infection and immunity · 2006Article
- Protein expression profiles of Chlamydia pneumoniae in models of persistence versus those of heat shock stress response.Infection and immunity · 2006Article
- Effects of repeated Chlamydia pneumoniae inoculations on aortic lipid accumulation and inflammatory response in C57BL/6J mice.Infection and immunity · 2005Article
- Interaction between components of the type III secretion system of Chlamydiaceae.Journal of bacteriology · 2005Article
- Genomic-scale analysis of bacterial gene and protein expression in the host.Emerging infectious diseases · 2004Review
- Chlamydial persistence: beyond the biphasic paradigm.Infection and immunity · 2004Review
- Temporal expression of type III secretion genes of Chlamydia pneumoniae.Infection and immunity · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent data have shown that the respiratory pathogen Chlamydia pneumoniae expresses an altered gene transcription profile during gamma interferon (IFN-gamma)-induced persistent infection in vitro. In the present study, we examined, by proteomics, expression of C. pneumoniae proteins labeled intracellularly with [(35)S]methionine/cysteine under normal conditions or IFN-gamma-mediated persistence. The identity of differentially expressed proteins during persistent infection was determined by matching spots to those of proteins identified in C. pneumoniae elementary bodies by matrix-assisted laser desorption ionization mass spectrometry. Upon treatment with 50 U of IFN-gamma per ml, a marked upregulation of major outer membrane protein (MOMP), heat shock protein 60 (Hsp-60/GroEL), and proteins with functions in DNA replication (GyrA), transcription (RpoA, PnP), translation (Rrf), glycolysis (PgK, GlgP), and type III secretion (SctN) was observed at 24 h of infection. In contrast, no significant decreases in bacterial protein expression were found in C. pneumoniae-infected cells due to IFN-gamma treatment. Upregulation of C. pneumoniae proteins involved in diverse functions during persistent infection may allow the organism to resist the inhibitory effects of IFN-gamma while retaining basic functions. Future studies should examine the differential expression of chlamydial proteins during the developmental cycle under IFN-gamma pressure to obtain a finer representation of the gene products involved in establishing persistence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.