ArticleMolecular biology of the cell2002
Symplekin, a constitutive protein of karyo- and cytoplasmic particles involved in mRNA biogenesis in Xenopus laevis oocytes.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 90 citations in OpenAlex.
- Molecular details of the CPSF73-CPSF100 C-terminal heterodimer and interaction with Symplekin.Open biology · 2023Article
- SYMPK Is Required for Meiosis and Involved in Alternative Splicing in Male Germ Cells.Frontiers in cell and developmental biology · 2021Article
- Integrator subunit 4 is a 'Symplekin-like' scaffold that associates with INTS9/11 to form the Integrator cleavage module.Nucleic acids research · 2018Article
- Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.Molecular and cellular biology · 2014Review
- Nuclear ARVCF protein binds splicing factors and contributes to the regulation of alternative splicing.The Journal of biological chemistry · 2014Article
- Drosophila Symplekin localizes dynamically to the histone locus body and tricellular junctions.Nucleus (Austin, Tex.) · 2014Article
- Labeling primary amine groups in peptides and proteins with N-hydroxysuccinimidyl ester modified Fe3O4@SiO2 nanoparticles containing cleavable disulfide-bond linkers.Bioconjugate chemistry · 2013Article
- Non-canonical Cajal bodies form in the nucleus of late stage avian oocytes lacking functional nucleolus.Histochemistry and cell biology · 2012Article
- CPEB4 is a cell survival protein retained in the nucleus upon ischemia or endoplasmic reticulum calcium depletion.Molecular and cellular biology · 2010Article
- Symplekin specifies mitotic fidelity by supporting microtubule dynamics.Molecular and cellular biology · 2010Article
- Desmosomal plakophilins in the prostate and prostatic adenocarcinomas: implications for diagnosis and tumor progression.The American journal of pathology · 2010Article
- Symplekin promotes tumorigenicity by up-regulating claudin-2 expression.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Protein p0071, an armadillo plaque protein of adherens junctions, is predominantly expressed in distal renal tubules.Histochemistry and cell biology · 2010Article
- Junctional music that the nucleus hears: cell-cell contact signaling and the modulation of gene activity.Cold Spring Harbor perspectives in biology · 2009Review
- Differential expression pattern of protein ARVCF in nephron segments of human and mouse kidney.Histochemistry and cell biology · 2008Article
- Translational control by cytoplasmic polyadenylation in Xenopus oocytes.Biochimica et biophysica acta · 2008Review
- Sumoylation modulates the assembly and activity of the pre-mRNA 3' processing complex.Molecular and cellular biology · 2007Article
- U2 snRNP binds intronless histone pre-mRNAs to facilitate U7-snRNP-dependent 3' end formation.Molecular cell · 2007Article
- Review
- Characterization of mammalian Par 6 as a dual-location protein.Molecular and cellular biology · 2007Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Symplekin is a dual location protein that has been localized to the cytoplasmic plaques of tight junctions but also occurs in the form of interchromatin particles in the karyoplasm. Here we report the identification of two novel and major symplekin-containing protein complexes in both the karyo- and the cytoplasm of Xenopus laevis oocytes. Buffer-extractable fractions from the karyoplasm of stage IV-VI oocytes contain an 11S particle, prepared by immunoselection and sucrose gradient centrifugation, in which symplekin is associated with the subunits of the cleavage and polyadenylation specificity factor (CPSF). Moreover, in immunofluorescence microscopy nuclear symplekin colocalizes with protein CPSF-100 in the "Cajal bodies." However, symplekin is also found in cytoplasmic extracts of enucleated oocytes and egg extracts, where it occurs in 11S as well as in ca. 65S particles, again in association with CPSF-100. This suggests that, in X. laevis oocytes, symplekin is possibly involved in both processes, 3'-end processing of pre-mRNA in the nucleus and regulated polyadenylation in the cytoplasm. We discuss the possible occurrence of similar symplekin-containing particles involved in mRNA metabolism in the nucleus and cytoplasm of other kinds of cells, also in comparison with the nuclear forms of other dual location proteins in nuclei and cell junctions.
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