ArticleMolecular biology of the cell2002
Selective and signal-dependent recruitment of membrane proteins to secretory granules formed by heterologously expressed von Willebrand factor.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 67 citations in OpenAlex.
- Endothelial cells expressing CPE and vWF are involved in the Immunopathogenesis of primary biliary cholangitis.Scientific reports · 2025Article
- The winding road to platelet α-granules.Frontiers in cell and developmental biology · 2025Review
- P-selectin mobility undergoes a sol-gel transition as it diffuses from exocytosis sites into the cell membrane.Nature communications · 2022Article
- Targeting transgenic proteins to alpha granules for platelet-directed gene therapy.Molecular therapy. Nucleic acids · 2022Article
- Article
- Fibroblastic Reticular Cells Control Conduit Matrix Deposition during Lymph Node Expansion.Cell reports · 2019Article
- Von Willebrand Disease: From In Vivo to In Vitro Disease Models.HemaSphere · 2019Review
- Synaptotagmin 5 regulates CaJournal of cell science · 2019Article
- Tuning the endothelial response: differential release of exocytic cargos from Weibel-Palade bodies.Journal of thrombosis and haemostasis : JTH · 2018Article
- Review
- von Willebrand factor multimerization and the polarity of secretory pathways in endothelial cells.Blood · 2016Article
- Type II PI4-kinases control Weibel-Palade body biogenesis and von Willebrand factor structure in human endothelial cells.Journal of cell science · 2016Article
- Syntaxin-binding protein STXBP5 inhibits endothelial exocytosis and promotes platelet secretion.The Journal of clinical investigation · 2014Article
- Self-assembly of VPS41 promotes sorting required for biogenesis of the regulated secretory pathway.Developmental cell · 2013Article
- Actomyosin II contractility expels von Willebrand factor from Weibel-Palade bodies during exocytosis.The Journal of cell biology · 2011Article
- RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway.The Journal of cell biology · 2010Article
- Protein mobilities and P-selectin storage in Weibel-Palade bodies.Journal of cell science · 2010Article
- Role of lipid metabolism in smoothened derepression in hedgehog signaling.Developmental cell · 2010Article
- Article
- Platelet alpha-granules: basic biology and clinical correlates.Blood reviews · 2009Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
von Willebrand factor (vWF) is a large, multimeric protein secreted by endothelial cells and involved in hemostasis. When expressed in AtT-20 cells, vWF leads to the de novo formation of cigar-shaped organelles similar in appearance to the Weibel-Palade bodies of endothelial cells in which vWF is normally stored before regulated secretion. The membranes of this vWF-induced organelle, termed the pseudogranule, are uncharacterized. We have examined the ability of these pseudogranules, which we show are secretagogue responsive, to recruit membrane proteins. Coexpression experiments show that the Weibel-Palade body proteins P-selectin and CD63, as well as the secretory organelle membrane proteins vesicle-associated membrane protein-2 and synaptotagmin I are diverted away from the endogenous adrenocorticotropic hormone-containing secretory granules to the vWF-containing pseudogranules. However, transferrin receptor, lysosomal-associated membrane protein 1, and sialyl transferase are not recruited. The recruitment of P-selectin is dependent on a tyrosine-based motif within its cytoplasmic domain. Our data show that vWF pseudogranules specifically recruit a subset of membrane proteins, and that in a process explicitly driven by the pseudogranule content (i.e., vWF), the active recruitment of at least one component of the pseudogranule membrane (i.e., P-selectin) is dependent on residues of P-selectin that are cytosolic and therefore unable to directly interact with vWF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.