ArticleMolecular biology of the cell2002
SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 67 citations in OpenAlex.
- Sld3CBD-Cdc45 structural insights into Cdc45 recruitment for CMG complex formation during DNA replication.eLife · 2025Article
- Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation.The EMBO journal · 2016Article
- Consequences of abnormal CDK activity in S phase.Cell cycle (Georgetown, Tex.) · 2016Article
- The Replication Initiation Protein Sld3/Treslin Orchestrates the Assembly of the Replication Fork Helicase during S Phase.The Journal of biological chemistry · 2015Article
- Evolutionary conservation of the CDK targets in eukaryotic DNA replication initiation.Chromosoma · 2015Review
- Human DNA helicase B interacts with the replication initiation protein Cdc45 and facilitates Cdc45 binding onto chromatin.Experimental cell research · 2015Article
- Switch on the engine: how the eukaryotic replicative helicase MCM2-7 becomes activated.Chromosoma · 2015Review
- Helicase activation and establishment of replication forks at chromosomal origins of replication.Cold Spring Harbor perspectives in biology · 2013Review
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- Efficient initiation of DNA replication in eukaryotes requires Dpb11/TopBP1-GINS interaction.Molecular and cellular biology · 2013Article
- The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.The Journal of biological chemistry · 2013Article
- DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.Molecular biology of the cell · 2012Article
- Treslin, DUE-B, and GEMC1 cannot complement Sld3 mutants in fission yeast.FEMS yeast research · 2012Article
- Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.The EMBO journal · 2012Article
- CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast.Molecular biology of the cell · 2011Article
- Auxin-inducible protein depletion system in fission yeast.BMC cell biology · 2011Article
- Review
- How do Cdc7 and cyclin-dependent kinases trigger the initiation of chromosome replication in eukaryotic cells?Genes & development · 2010Review
- GEMC1 is a TopBP1-interacting protein required for chromosomal DNA replication.Nature cell biology · 2010Article
- The DNA unwinding element binding protein DUE-B interacts with Cdc45 in preinitiation complex formation.Molecular and cellular biology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Initiation of DNA replication in eukaryotic cells is regulated through the ordered assembly of replication complexes at origins of replication. Association of Cdc45 with the origins is a crucial step in assembly of the replication machinery, hence can be considered a target for the regulation of origin activation. To examine the process required for SpCdc45 loading, we isolated fission yeast SpSld3, a counterpart of budding yeast Sld3 that interacts with Cdc45. SpSld3 associates with the replication origin during G1-S phases and this association depends on Dbf4-dependent (DDK) kinase activity. In the corresponding period, SpSld3 interacts with minichromosome maintenance (MCM) proteins and then with SpCdc45. A temperature-sensitive sld3-10 mutation suppressed by the multicopy of the sna41+ encoding SpCdc45 impairs loading of SpCdc45 onto chromatin. In addition, this mutation leads to dissociation of preloaded Cdc45 from chromatin in the hydroxyurea-arrested S phase, and DNA replication upon removal of hydroxyurea is retarded. Thus, we conclude that SpSld3 is required for stable association of Cdc45 with chromatin both in initiation and elongation of DNA replication. The DDK-dependent origin association suggests that SpSld3 is involved in temporal regulation of origin firing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.