ArticleThe Journal of biological chemistry2002
Use of an in vivo reporter assay to test for transcriptional and translational fidelity in yeast.
Article in The Journal of biological chemistry, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 62 citations in OpenAlex.
- Phenotypic mutations contribute to protein diversity and shape protein evolution.Protein science : a publication of the Protein Society · 2022Review
- EGFP Reporters for Direct and Sensitive Detection of Mutagenic Bypass of DNA Lesions.Biomolecules · 2020Article
- Article
- The Mechanisms of Substrate Selection, Catalysis, and Translocation by the Elongating RNA Polymerase.Journal of molecular biology · 2019Review
- Transcription fidelity and its roles in the cell.Current opinion in microbiology · 2018Review
- The landscape of transcription errors in eukaryotic cells.Science advances · 2017Article
- AGenetics · 2017Article
- Misincorporation by RNA polymerase is a major source of transcription pausing in vivo.Nucleic acids research · 2017Article
- Genome stability versus transcript diversity.DNA repair · 2016Review
- Article
- A genetic assay for transcription errors reveals multilayer control of RNA polymerase II fidelity.PLoS genetics · 2014Article
- High level constitutive expression of luciferase reporter by lsd90 promoter in fission yeast.PloS one · 2014Article
- Large-scale detection of in vivo transcription errors.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Direct assessment of transcription fidelity by high-resolution RNA sequencing.Nucleic acids research · 2013Article
- The fidelity of transcription: RPB1 (RPO21) mutations that increase transcriptional slippage in S. cerevisiae.The Journal of biological chemistry · 2013Article
- Isolation and characterization of RNA polymerase rpoB mutations that alter transcription slippage during elongation in Escherichia coli.The Journal of biological chemistry · 2013Article
- Isolation and characterization of transcription fidelity mutants.Biochimica et biophysica acta · 2012Review
- Preventing dangerous nonsense: selection for robustness to transcriptional error in human genes.PLoS genetics · 2011Article
- Transcriptional mutagenesis: causes and involvement in tumour development.Nature reviews. Cancer · 2011Review
- Evolution of the mutation rate.Trends in genetics : TIG · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Eukaryotic RNA polymerase II and Escherichia coli RNA polymerase possess an intrinsic ribonuclease activity that is stimulated by the polymerase-binding proteins SII and GreB, respectively. This factor-activated hydrolysis of nascent RNA has been postulated to be involved in transcription elongation as well as removal of incorrect bases misincorporated into RNA. Little is known about the frequency of misincorporation by RNA polymerases in vivo or about the mechanisms involved in improving RNA polymerase accuracy. Here we have developed a luciferase reporter system in an effort to assay for base misincorporation in living Saccharomyces cerevisiae. The assay employs a luciferase open reading frame that contains a premature stop codon. The inactive truncated enzyme would become active if misincorporation by RNA polymerase II took place at the stop triplet. Yeast lacking SII did not display a significant change in reporter activity when compared with wild-type cells. We estimate that under our assay conditions, mRNAs with a misincorporation at the test site could not exceed 1 transcript per 500 cells. The reporter assay was very effective in detecting the previously described process of nonsense suppression (translational read-through) by ribosomes, making it difficult to determine an absolute level of basal (SII-independent) misincorporation by RNA polymerase II. Although these data cannot exclude the possibility that SII is involved in proofreading, they make it unlikely that such a contribution is physiologically significant, especially relative to the high frequency of translational errors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.