ArticleJournal of bacteriology2002
Construction and characterization of a cheA mutant of Treponema denticola.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
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- The Role ofFrontiers in cellular and infection microbiology · 2019Article
- Behavior of two Tannerella forsythia strains and their cell surface mutants in multispecies oral biofilms.Molecular oral microbiology · 2017Article
- Hypothetical Protein BB0569 Is Essential for Chemotaxis of the Lyme Disease Spirochete Borrelia burgdorferi.Journal of bacteriology · 2015Article
- Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola.Applied and environmental microbiology · 2015Article
- Inactivation of cyclic Di-GMP binding protein TDE0214 affects the motility, biofilm formation, and virulence of Treponema denticola.Journal of bacteriology · 2013Article
- Treponema denticola interactions with host proteins.Journal of oral microbiology · 2012Article
- Disruption of a type II endonuclease (TDE0911) enables Treponema denticola ATCC 35405 to accept an unmethylated shuttle vector.Applied and environmental microbiology · 2011Article
- CheY3 of Borrelia burgdorferi is the key response regulator essential for chemotaxis and forms a long-lived phosphorylated intermediate.Journal of bacteriology · 2011Article
- Virulence factors of the oral spirochete Treponema denticola.Journal of dental research · 2011Review
- Chemoreceptors and flagellar motors are subterminally located in close proximity at the two cell poles in spirochetes.Journal of bacteriology · 2011Article
- The diverse CheC-type phosphatases: chemotaxis and beyond.Molecular microbiology · 2008Article
- Identification of specific chemoattractants and genetic complementation of a Borrelia burgdorferi chemotaxis mutant: flow cytometry-based capillary tube chemotaxis assay.Applied and environmental microbiology · 2007Article
- Multiple functions of the leucine-rich repeat protein LrrA of Treponema denticola.Infection and immunity · 2004Article
- Diversity in chemotaxis mechanisms among the bacteria and archaea.Microbiology and molecular biology reviews : MMBR · 2004Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Treponema denticola cheA gene, encoding the central kinase of the general chemotaxis pathway, was analyzed for its role in chemotaxis and tissue penetration. The cheA gene was interrupted by insertion of an ermF-ermAM gene cassette. Reverse transcription-PCR confirmed that the other downstream chemotaxis genes within the same operon (cheW, cheX, and cheY) were still expressed in the cheA mutant strain. Lack of cheA resulted in decreased swarming on soft-agar swarm plates and failure to respond chemotactically to a mixture of nutrients. Behavioral analyses using video microscopy revealed that the cheA mutant exhibited coordinated cell movement. The cellular reversal frequency, however, was severely reduced, indicating that CheA in T. denticola mainly controls cellular reversal and that active chemotaxis signaling input is not required for coordination of flagellar rotation at both cell poles.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.