ArticleRNA (New York, N.Y.)2002
Switching the in vitro tRNA usage of HIV-1 by simultaneous adaptation of the PBS and PAS.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 37 citations in OpenAlex.
- Human T-cell leukemia virus type 1 uses a specific tRNARNA (New York, N.Y.) · 2024Article
- Retroviral PBS-segment sequence and structure: Orchestrating early and late replication events.Retrovirology · 2024Review
- Structural maturation of the HIV-1 RNA 5' untranslated region by Pr55RNA biology · 2022Article
- The three-way junction structure of the HIV-1 PBS-segment binds host enzyme important for viral infectivity.Nucleic acids research · 2021Article
- Extended Interactions between HIV-1 Viral RNA and tRNAInternational journal of molecular sciences · 2020Article
- Genetic variability of the U5 and downstream sequence of major HIV-1 subtypes and circulating recombinant forms.Scientific reports · 2020Article
- Distinct Conformational States Underlie Pausing during Initiation of HIV-1 Reverse Transcription.Journal of molecular biology · 2020Article
- Relating Structure and Dynamics in RNA Biology.Cold Spring Harbor perspectives in biology · 2019Review
- Role of host tRNAs and aminoacyl-tRNA synthetases in retroviral replication.The Journal of biological chemistry · 2019Review
- The human ortholog of archaeal Pus10 produces pseudouridine 54 in select tRNAs where its recognition sequence contains a modified residue.RNA (New York, N.Y.) · 2019Article
- Dynamic Interplay of RNA and Protein in the Human Immunodeficiency Virus-1 Reverse Transcription Initiation Complex.Journal of molecular biology · 2018Article
- RNase H sequence preferences influence antisense oligonucleotide efficiency.Nucleic acids research · 2017Article
- Heterogeneous structures formed by conserved RNA sequences within the HIV reverse transcription initiation site.RNA (New York, N.Y.) · 2016Article
- A structure-based mechanism for tRNA and retroviral RNA remodelling during primer annealing.Nature · 2014Article
- Small noncoding RNAs in cells transformed by human T-cell leukemia virus type 1: a role for a tRNA fragment as a primer for reverse transcriptase.Journal of virology · 2014Article
- Role of the primer activation signal in tRNA annealing onto the HIV-1 genome studied by single-molecule FRET microscopy.RNA (New York, N.Y.) · 2013Article
- The Interaction between tRNA(Lys) 3 and the primer activation signal deciphered by NMR spectroscopy.PloS one · 2013Article
- Initiation of HIV Reverse Transcription.Viruses · 2010Article
- The availability of the primer activation signal (PAS) affects the efficiency of HIV-1 reverse transcription initiation.Nucleic acids research · 2007Article
- Structural polymorphism of the HIV-1 leader region explored by computational methods.Nucleic acids research · 2005Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse transcription of the HIV-1 RNA genome is primed by the cellular tRNA(lys3) molecule that anneals to a complementary sequence in the viral genome, the primer-binding site (PBS). Additional interactions between the tRNA primer and the viral RNA were proposed to play a role in reverse transcription. We recently identified an 8-nt element in the U5 region upstream of the PBS that is critical for initiation and processive elongation of reverse transcription. This motif was termed the primer activation signal (PAS), and is proposed to interact with the "antiPAS sequence" in the TphiC arm of tRNA(lys3). In this study, we demonstrate that the efficiency of initiation of reverse transcription can be modulated by PAS mutations that strengthen or weaken the interaction with antiPAS. These results provide further evidence for a direct base-pairing interaction between the PAS in the viral RNA and the antiPAS in the tRNA(lys3) molecule. A broad phylogenetic survey indicated that a PAS element is present in all retroviral RNA genomes, suggesting that the process of reverse transcription is regulated by a common mechanism in all retroviridae. It has proven very difficult to change the identity of the tRNA primer for HIV-1 reverse transcription by changing the PBS sequence. Using in vitro reverse transcription assays, we demonstrate that the identity of the priming tRNA species can be switched by simultaneous alteration of the PBS and PAS motifs to accommodate a new tRNA primer. These results indicate that the PAS-antiPAS interaction is important for both primer selection and efficient reverse transcription.
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