ArticleRNA (New York, N.Y.)2002
Replacement of the yeast TRP4 3' untranslated region by a hammerhead ribozyme results in a stable and efficiently exported mRNA that lacks a poly(A) tail.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Asc1, Hel2, and Slh1 couple translation arrest to nascent chain degradation.RNA (New York, N.Y.) · 2017Article
- Release factor eRF3 mediates premature translation termination on polylysine-stalled ribosomes in Saccharomyces cerevisiae.Molecular and cellular biology · 2014Article
- Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm.Nucleic acids research · 2011Article
- Article
- Messenger RNA export from the nucleus: a series of molecular wardrobe changes.Traffic (Copenhagen, Denmark) · 2009Review
- A conserved role for cytoplasmic poly(A)-binding protein 1 (PABPC1) in nonsense-mediated mRNA decay.The EMBO journal · 2007Article
- 3'-end formation signals modulate the association of genes with the nuclear periphery as well as mRNP dot formation.The EMBO journal · 2006Article
- Yeast transcripts cleaved by an internal ribozyme provide new insight into the role of the cap and poly(A) tail in translation and mRNA decay.RNA (New York, N.Y.) · 2006Article
- A synthetic A tail rescues yeast nuclear accumulation of a ribozyme-terminated transcript.RNA (New York, N.Y.) · 2004Article
- A quantitative analysis of intron effects on mammalian gene expression.RNA (New York, N.Y.) · 2003Article
- Poly(A)-binding proteins: multifunctional scaffolds for the post-transcriptional control of gene expression.Genome biology · 2003Review
- Coupling of termination, 3' processing, and mRNA export.Molecular and cellular biology · 2002Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The mRNA poly(A) tail serves different purposes, including the facilitation of nuclear export, mRNA stabilization, efficient translation, and, finally, specific degradation. The posttranscriptional addition of a poly(A) tail depends on sequence motifs in the 3' untranslated region (3' UTR) of the mRNA and a complex trans-acting protein machinery. In this study, we have replaced the 3' UTR of the yeast TRP4 gene with sequences encoding a hammerhead ribozyme that efficiently cleaves itself in vivo. Expression of the TRP4-ribozyme allele resulted in the accumulation of a nonpolyadenylated mRNA. Cells expressing the TRP4-ribozyme mRNA showed a reduced growth rate due to a reduction in Trp4p enzyme activity. The reduction in enzyme activity was not caused by inefficient mRNA export from the nucleus or mRNA destabilization. Rather, analyses of mRNA association with polyribosomes indicate that translation of the ribozyme-containing mRNA is impaired. This translational defect allows sufficient synthesis of Trp4p to support growth of trp4 cells, but is, nevertheless, of such magnitude as to activate the general control network of amino acid biosynthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.