Evidence map›Paper›PMID 12003489›Full record

ArticleRNA (New York, N.Y.)2002

Purification and characterization of Rpp25, an RNA-binding protein subunit of human ribonuclease P.

Cecilia Guerrier-Takada, Paul S Eder, Venkat Gopalan, Sidney Altman

Open access · bronzeAbstract read
In one paragraph

Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 62 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Human RNase P exhibits and controls distinct ribonucleolytic activities required for ordered maturation of tRNA.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Characterization of ALBA Family Expression and Localization inInternational journal of molecular sciences · 2021
    Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Superantigens hyperinduce inflammatory cytokines by enhancing the B7-2/CD28 costimulatory receptor interaction.Proceedings of the National Academy of Sciences of the United States of America · 2016
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Cecilia Guerrier-TakadaDepartment of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
Paul S Eder
Venkat Gopalan
Sidney Altman
Yale University · US

Funding

TRNA: STUDIES OF PRECURSORS, MUTANTS AND FUNCTIONSR01GM019422 · NIGMS · YALE UNIVERSITY · PI ALTMAN, SIDNEY · 1985 to 2003
$2.4M
TRNA: STUDIES OF PRECURSORS, MUTANTS, AND FUNCTIONR37GM019422 · NIGMS · YALE UNIVERSITY · PI ALTMAN, SIDNEY · 1989 to 1999
–
NIGMS NIH HHS GM-19422
6 · The paper itself

Abstract

In HeLa cells, ribonuclease P (RNase P), the tRNA processing enzyme consists of an RNA subunit (H1 RNA) associated with at least nine protein subunits, Rpp14, Rpp20, Rpp21, Rpp29 (hPop4), Rpp30, Rpp38, Rpp40, hPop1, and hPop5 (18.8 kDa). We report here the cloning and immuno-biochemical analysis of Rpp25, another protein subunit of RNase P. Polyclonal rabbit antibodies raised against recombinant Rpp25 recognize their corresponding antigens in RNase P-containing fractions purified from HeLa cells, and they also precipitate active holoenzyme. Furthermore, this protein has general RNA binding properties.

Indexed as

Amino Acid SequenceBase SequenceBlotting, WesternCloning, MolecularDNA PrimersElectrophoretic Mobility Shift AssayEndoribonucleasesHeLa CellsHumansMolecular Sequence DataPolymerase Chain ReactionPrecipitin TestsProtein SubunitsRibonuclease PRNA-Binding ProteinsRNA, CatalyticDNA PrimersEndoribonucleasesProtein SubunitsRibonuclease PRNA-Binding ProteinsRNA, CatalyticRPP14 protein, humanRPP25 protein, human

Identifiers

PMID12003489
PMCPMC1370251
OpenAlexW2163909810

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.