ArticleNucleic acids research2002
High affinity nucleic acid aptamers for streptavidin incorporated into bi-specific capture ligands.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 66 citations in OpenAlex.
- Bone marrow mesenchymal stem cells derived exosomal miRNAs can modulate diabetic bone-fat imbalance.Frontiers in endocrinology · 2023Article
- In vitro selection of aptamers and their applications.Nature reviews. Methods primers · 2023Article
- Roles ofMicroorganisms · 2022Review
- Recent advances in aptamer applications for analytical biochemistry.Analytical biochemistry · 2022Article
- Tagging and Capturing of Lentiviral Vectors Using Short RNAs.International journal of molecular sciences · 2021Article
- Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices.Sensors (Basel, Switzerland) · 2021Review
- Nanoscale delivery systems for microRNAs in cancer therapy.Cellular and molecular life sciences : CMLS · 2020Review
- Bispecific therapeutic aptamers for targeted therapy of cancer: a review on cellular perspective.Journal of molecular medicine (Berlin, Germany) · 2018Review
- Identification and characterization of nucleobase-modified aptamers by click-SELEX.Nature protocols · 2018Article
- Circular siRNAs for Reducing Off-Target Effects and Enhancing Long-Term Gene Silencing in Cells and Mice.Molecular therapy. Nucleic acids · 2018Article
- Multivalent Aptamers: Versatile Tools for Diagnostic and Therapeutic Applications.Molecules (Basel, Switzerland) · 2016Review
- Integrated Microfluidic Isolation of Aptamers Using Electrophoretic Oligonucleotide Manipulation.Scientific reports · 2016Article
- MicroRNA-188 regulates age-related switch between osteoblast and adipocyte differentiation.The Journal of clinical investigation · 2015Article
- High efficiency acetylcholinesterase immobilization on DNA aptamer modified surfaces.Molecules (Basel, Switzerland) · 2014Article
- Article
- Chimeric aptamers in cancer cell-targeted drug delivery.Critical reviews in biochemistry and molecular biology · 2011Review
- Bi-specific aptamers mediating tumor cell lysis.The Journal of biological chemistry · 2011Article
- Methods for measuring aptamer-protein equilibria: a review.Analytica chimica acta · 2011Review
- Upgrading SELEX technology by using lambda exonuclease digestion for single-stranded DNA generation.Molecules (Basel, Switzerland) · 2009Article
- Structural characterization of an anti-gp120 RNA aptamer that neutralizes R5 strains of HIV-1.RNA (New York, N.Y.) · 2005Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated 2'-Fluoro-substituted RNA aptamers that bind to streptavidin (SA) with an affinity around 7 +/- 1.8 nM, comparable with that of recently described peptide aptamers. Binding to SA was not prevented by prior saturation with biotin, enabling nucleic acid aptamers to form useful ternary complexes. Mutagenesis, secondary structure analysis, ribonuclease footprinting and deletion analysis provided evidence for the essential structural features of SA-binding aptamers. In order to provide a general method for the exploitation of these aptamers, we produced derivatives in which they were fused to the naturally structured RNA elements, CopT or CopA. In parallel, we produced derivatives of CD4-binding aptamers fused to the complementary CopA or CopT elements. When mixed, these two chimeric aptamers rapidly hybridized, by virtue of CopA-CopT complementarity, to form stable, bi-functional aptamers that we called 'adaptamers'. We show that a CD4-SA-binding adaptamer can be used to capture CD4 onto a SA-derivatized surface, illustrating their general utility as indirect affinity ligands.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.